Study type: Medical/biological study (experimental study)

Effects on G2/M phase cell cycle distribution and aneuploidy formation of exposure to a 60 Hz electromagnetic field in combination with ionizing radiation or hydrogen peroxide in L132 nontumorigenic human lung epithelial cells. med./bio.

Published in: Korean J Physiol Pharmacol 2015; 19 (2): 119-124

Aim of study (acc. to author)

The effects of exposure of human lung epithelial cells to a 60 Hz magnetic field alone or with co-exposure to gamma radiation or hydrogen peroxide on the cell cycle distribution and on aneuploidy should be investigated.

Background/further details

Cells were divided into the following groups: 1) exposure to a 1 mT magnetic field, 2) exposure to a 2 mT magnetic field, 3) exposure to hydrogen peroxide from the beginning of the exposure phase (0.05, 0.1, 0.5 mM), 4) exposure to gamma radiation at the beginning of the exposure phase (0.1, 0.5, 1, 2 Gy), 5) co-exposure to 2 mT magnetic field and 0.05 mM hydrogen peroxide simultaneously and 6) co-exposure to 2 mT magnetic field and 0.5 Gy gamma radiation (prior to magnetic field exposure). For each group and test, a separate sham exposure was conducted.
Each test was performed in triplicate and at least in four independent experiments.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: continuous for 9 hours
Exposure 2: 60 Hz
Exposure duration: continuous for 9 hours

General information

gamma radiation was applied as a single dose at the beginning of each exposure time, respectively, at a rate of 5 Gy/min

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 9 hours
Exposure setup
Exposure source
Chamber 60 mm cell culture dishes
Setup temperature was maintained at 37 ± 0.3°C
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - - - -

Exposure 2

Main characteristics
Frequency 60 Hz
Type
Exposure duration continuous for 9 hours
Exposure setup
Exposure source
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 2 mT - - - -

Reference articles

  • Lee HJ et al. (2012): Combined effects of 60 Hz electromagnetic field exposure with various stress factors on cellular transformation in NIH3T3 cells.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No significant changes could be found between magnetic field exposed (groups 1 and 2) and sham exposed cells.
In groups 3 (hydrogen peroxide) and 4 (gamma radiation), significant changes in the cell cycle distribution and aneuploidy could be detected between exposed and sham exposed cells.
Exposure to the magnetic field and co-exposure to hydrogen peroxide (group 5) or gamma radiation (group 6) did not result in an enhancement of the effects of hydrogen peroxide or gamma radiation alone.
The authors conclude that exposure of human lung epithelial cells to a 60 Hz magnetic field has no effect on the cell cycle distribution and aneuploidy and does not enhance the effects of gamma radiation or hydrogen peroxide.

Study character:

Study funded by

Related articles