研究のタイプ: 医学/生物学の研究 (experimental study)

[非腫瘍形成性ヒト細胞株における60Hz磁界の電離放射線との組合せばく露によるγ-H2AXの増加、ただし過酸化水素との組合せでは増加しない] med./bio.

Increased gamma-H2AX by exposure to a 60-Hz magnetic fields combined with ionizing radiation, but not hydrogen peroxide, in non-tumorigenic human cell lines.

掲載誌: Int J Radiat Biol 2014; 90 (4): 291-298

この研究は、非腫瘍形成性ヒト細胞株(ヒト肺線維芽細胞WI-38およびヒト肺上皮細胞L132)において、60Hz磁界MF)と電離放射線(IR)またはH2O2との組合せばく露がDNA損傷反応に影響するか否かを調べた。MF(0、1、2 mT)への6時間ばく露、IR(0、0.5、1、2 Gyのガンマ線)への1回ばく露後6時間培養、またはH2O2(0、0.1、0.5、1 mM)添加後に6時間培養のそれぞれ単独ばく露の他、組合せばく露として、MF+IR(2 mT+1 Gy)、MFH2O2(2 mT+0.05または1 mM)の6時間ばく露を行い、リン酸化ヒストン2AX(γ-H2AX)発現およびγ-H2AXフォーカス生成(DNA2重鎖切断マーカ)を調べた。その結果、磁界単独では、無ばく露対照に比べ、2 mTでγ-H2AX発現とγ-H2AXフォーカス生成の増加が見られたが、1 mTでは見られなかった;MF+IRばく露の場合、IR単独に比べ、γ-H2AX発現とγ-H2AXフォーカス生成の増加が高まった;MF+H2O2ばく露では、H2O2単独との差が無かった、と報告している。

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研究目的(著者による)

To examine whether extremely low frequency magnetic fieldsalone and in combination with ionizing radiation or hydrogen peroxide (H2O2) induce DNA damage in human lung fibroblasts and human lung epithelial cells.

詳細情報

Cells were subjected to different treatments: 1.) control group, 2.) magnetic field exposure alone (1 mT or 2 mT), 3.) magnetic field exposure (2 mT) in the presence of H2O2 (fibroblasts: 0.05mM; epithelial cells: 1 mM) and 4.) magnetic field exposure (2 mT) and afterwards radiation with gamma rays (1 Gy). As positive controls, the cells were treated with different doses of gamma rays (single dose 0-2 Gy, dose rate of 5 Gy/min) or cultivated with different concentrations of H2O2 (0-1 mM) for 6 hours.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 6 hours
magnetic field alone
ばく露2: 60 Hz
ばく露時間: continuous for 6 hours
magnetic field + ionizing radiation
ばく露3: 60 Hz
ばく露時間: continuous for 6 hours
magnetic field + hydrogen peroxide

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 6 hours
Additional information magnetic field alone
ばく露装置
ばく露の発生源/構造
  • 詳細不明
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -
磁束密度 2 mT - - - -

ばく露2

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 6 hours
Additional information magnetic field + ionizing radiation
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - - - -

ばく露3

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 6 hours
Additional information magnetic field + hydrogen peroxide
ばく露装置
チャンバの詳細 cells were cultivated and exposed in 60 mm cell culture dishes
ばく露装置の詳細 temperature in exposure chamber was 37°C ± 0.3°C
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT - - - -

Reference articles

  • Lee HJ et al. (2012): [NIH3T3細胞における細胞の形質転換に対する各種のストレス要因と60Hz電磁界ばく露の複合的な影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In both cell lines, exposure to a magnetic field alone with the intensity of 2 mT led to a significant increase in DNA double strand breaks compared to the control group, while a magnetic field with the intensity of 1 mT had no significant effect. The combination of magnetic field exposure and gamma radiation led to significant more DNA double strand breaks in both cell lines than one of the treatments alone, while no synergistic effect was found regarding the combination of magnetic field exposure and H2O2.
The authors conclude that extremely low frequency magnetic fields could induce DNA damage in human lung fibroblasts and human lung epithelial cells. Additionally, the data indicate that magnetic field exposure and gamma radiation could act synergistically.

研究の種別:

研究助成

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