研究のタイプ: 医学/生物学の研究 (experimental study)

[パルス化電磁界は一次繊毛の存在を必要とする骨芽細胞の無機質化および成熟を促進する] med./bio.

Pulsed electromagnetic fields promote osteoblast mineralization and maturation needing the existence of primary cilia.

掲載誌: Mol Cell Endocrinol 2015; 404: 132-140

この研究は、培養された頭頂骨の骨芽細胞増殖および骨形成分化に対する50Hzパルス電磁界ばく露(PEMFs:1日90分間)の影響を調べた。PEMFsの強度を0.6-3.6mTの範囲で0.6mT刻みで変えた。「一次繊毛にPEMFセンサ機能がある」という仮説を立て、その検証も合わせて実施した。その結果、全ての磁界強度増殖への影響が見られたが、0.6mT群で最も影響が大きかった;0.6mT群では骨形成効果も最も大きかった;IFT88のsiRNA導入により一次繊毛を抑制した場合、0.6mTでのPEMFsの骨形成促進効果が消失した、と報告している。

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研究目的(著者による)

The effects of exposure of rat calvarial osteoblasts to a pulsed 50 Hz magnetic field on cell proliferation and differentiation as well as and the role of primary cilia in this context should be investigated.

詳細情報

Cells were divided into the following groups: exposure to a magnetic field with 1) 0.6 mT, 2) 1.2 mT, 3) 1.8 mT, 4) 2.4 mT, 5) 3.0 mT, 6) 3.6 mT and 7) sham exposure. Additionally, cells with a block in primary cilium formation were created via transfection by siRNA and a resulting suppressed expression of the intraflagellar transport (IFT) protein 88. These cells were exposed to a 0,6 mT magnetic field (group 8).
Each experiment was conducted with n=3 samples per group and was repeated 3 times.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
  • DC/static
ばく露時間: continuous for 90 minutes/day for up to 12 days
ばく露2:
  • DC/static
Modulation type: pulsed
ばく露時間: continuous for 90 minutes/day for up to 12 days
ばく露3:
  • DC/static
Modulation type: pulsed
ばく露時間: continuous for 90 minutes/day for up to 12 days
ばく露4:
  • DC/static
Modulation type: pulsed
ばく露時間: continuous for 90 minutes/day for up to 12 days
ばく露5:
  • DC/static
Modulation type: pulsed
ばく露時間: continuous for 90 minutes/day for up to 12 days
ばく露6:
  • DC/static
Modulation type: pulsed
ばく露時間: continuous for 90 minutes/day for up to 12 days

ばく露1

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 continuous for 90 minutes/day for up to 12 days
Additional information 50 Hz pulsed unipolar magnetic field with 50% duty ratio
ばく露装置
ばく露の発生源/構造
チャンバの詳細 60-mm culture dishes
ばく露装置の詳細 solenoid (27 cm long, three series-connected coils and lead tube core cylinder with inner diameter of 100 mm) was placed in a CO2 incubator (5% CO2, 37 °C and 100% humidity); fields had an error of <0.1% horizontally (from 60 to -60 mm) and <0.05% vertically (from 45 to -45 mm)
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.6 mT - 測定値 - -

ばく露2

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 continuous for 90 minutes/day for up to 12 days
Additional information 50 Hz pulsed unipolar magnetic field with 50% duty ratio
Modulation
Modulation type pulsed
Duty cycle 50 %
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.2 mT - 測定値 - -

ばく露3

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 continuous for 90 minutes/day for up to 12 days
Additional information 50 Hz pulsed unipolar magnetic field with 50% duty ratio
Modulation
Modulation type pulsed
Duty cycle 50 %
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.8 mT - 測定値 - -

ばく露4

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 continuous for 90 minutes/day for up to 12 days
Additional information 50 Hz pulsed unipolar magnetic field with 50% duty ratio
Modulation
Modulation type pulsed
Duty cycle 50 %
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2.4 mT - 測定値 - -

ばく露5

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 continuous for 90 minutes/day for up to 12 days
Additional information 50 Hz pulsed unipolar magnetic field with 50% duty ratio
Modulation
Modulation type pulsed
Duty cycle 50 %
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 3 mT - 測定値 - -

ばく露6

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 continuous for 90 minutes/day for up to 12 days
Additional information 50 Hz pulsed unipolar magnetic field with 50% duty ratio
Modulation
Modulation type pulsed
Duty cycle 50 %
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 3.6 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Cell proliferation was significantly increased in groups 1-6 compared to the sham exposure group with the most distinct increase in group 1 (0.6 mT).
The parameters for differentiation and mineralization also showed the highest and most distinctly significantly increased values in group 1 in comparison to the sham exposure group without exceptions.
Differentiation and mineralization were significantly reduced in group 8 compared to group 1 and no significant differences could be found compared to the sham exposure group.
The authors conclude that exposure of rat calvarial osteoblasts to a pulsed 50 Hz magnetic field with 0.6 mT might stimulate cell proliferation, differentiation and mineralization and that the primary cilium plays an essential role in mediating this effects.

研究の種別:

研究助成

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