Twenty rats were randomly and evenly divided into two groups: an exposure group and a sham exposure group. At the end of exposure, the rats were fasted for 24 hours and euthanized and the brains were removed.
Note: Further groups with radiofrequency exposure are mentioned in the method section, however, their results are not presented in this article.
|Exposure duration||22 hours per day for 28 days|
|Setup||plastic cage was positioned between two electrodes placed 50 centimeters apart; one electrode received a potential of 5 kV from a high-voltage transformer; the cage housing the rats was placed on the ground electrode; the cage did not impede the electric field and allowed the free movement of the rats|
|Sham exposure||A sham exposure was conducted.|
|Additional info||temperature in the cage remained constant at 21°C, humidity remained constant and 24-hour circadian rhythm was maintained|
Total oxidative status and lipid peroxidation did not reveal any significant differences between the exposure group and the sham exposure group in different brain regions. Thus, the authors conclude that exposure to the electric field did not increase oxidative stress in the rat brain.
The enzyme activities of individual enzymes in the different brain regions of exposed rats were partially significantly different (mostly reduced) compared to the sham exposure group. However, the effects were not consistent between different brain regions.
The authors conclude that exposure of rats to a 50 Hz electric field does not affect oxidative stress in the investigated brain regions.