Study type: Medical/biological study (experimental study)

Antioxidants alleviate electric field-induced effects on lung tissue based on assays of heme oxygenase-1, protein carbonyl content, malondialdehyde, nitric oxide, and hydroxyproline. med./bio.

Published in: Sci Total Environ 2009; 407 (4): 1326-1332

Aim of study (acc. to author)

1) To study wether 50 Hz electric fields (12 kV/m) induce nitrosative stress and oxidative stress in guinea pigs lung tissue
2) To study the possible effects of extremely low frequency electric fields on collagen synthesis.

Background/further details

Additionally, the effects of antioxidant treatment such as epigallocatechin-3-gallate and N-acetyl-L-cysteine were investigated.
60 guinea pigs were divided into six groups: 1) control group (with saline injection), 2) epigallocatechin-3-gallate injection alone, 3) N-acetyl-L-cysteine injection alone, 4) saline injection before exposure, 5) epigallocatechin-3-gallate injection before exposure, and 6) N-acetyl-L-cysteine before exposure.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 8 h/day for 7 days

General information

animals were divided into 6 groups: i) control ii) injection of 25 mg/kg EGCG iii) injection of 300 mg/kg NAC iv) EMF exposure v) injection of EGCG + EMF exposure vi) injection of NAG + EMF exposure

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Exposure duration 8 h/day for 7 days
Exposure setup
Exposure source
  • 80 cm x 80 cm copper plate capacitor
Setup copper plates mounted on the top and bottom of the wooden cage
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 12 kV/m - measured and calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

While protein carbonyl content levels significantly increased, insignificant changes were observed in heme oxygenase-1, malondialdehyde, and nitric oxide levels for electric field exposure groups compared to the control group. The data showed no significant changes in these parameters on the electric field group compared to the group where epigallocatechin-3-gallate and N-acetyl-L-cysteine were separately applied along with electric field.

Study character:

Study funded by

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