Study type: Medical/biological study (experimental study)

Cytogenetic studies in human blood lymphocytes exposed in vitro to radiofrequency radiation at a cellular telephone frequency (835.62 MHz, FDMA). med./bio.

Published in: Radiat Res 2001; 155 (1): 113-121

Aim of study (acc. to editor)

Aim of the study was to investigate the extent of possible genetic damage caused by radiofrequency irradiation (835.62 MHz, 24 h, FDMA) on human blood lymphocytes in vitro.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 835.62 MHz
Modulation type: CW
Exposure duration: continuous for 24 h

Exposure 1

Main characteristics
Frequency 835.62 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for 24 h
Modulation
Modulation type CW
Additional info

FDMA/CW

Exposure setup
Exposure source
Chamber The facility housed nine RTLs (radial transmission lines) mounted on two separate adjacent sliding shelves. The facility and RTLs were shielded to avoid interference. Each RTL was formed by two parallel horizontal metal plates 4.3 cm apart, with a lightweight composite top plate hinged to the bottom aluminium plate (0.6 cm thick) providing mechanical support and homogeneity of the flasks' temperatures. The waves travelling from the central conical antenna were terminated by an annulus of MW-absorbing foam backed by perforated aluminium. The total radius was 53.3 cm.
Setup Each RTL was fully loaded with 16 T-75 culture flasks at a radius of 29.2 cm each containing 40 ml of liquid medium (about 5 mm depth) (two of them diluted blood). The distance of the cells settled at the bottom of the flask from the antenna was between 24.7 cm and 33.8 cm.
Additional info Aliquots of blood that were sham-exposed or exposed to 1.5 Gy of γ radiation were used as negative or positive controls.
Parameters
Measurand Value Type Method Mass Remarks
power 68 W - - - net forward power
power density 860 W/m² - - - at the center of the flask
SAR 4.4 W/kg mean measured and calculated - -
SAR 5 W/kg mean measured and calculated - -

Reference articles

  • Moros EG et al. (1999): The radial transmission line as a broad-band shielded exposure system for microwave irradiation of large numbers of culture flasks.

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The overall cytogenetic responses of the lymphocytes were similar although the absolute values were slightly different. The mitotic indices, the indices of abnormal cells showing chromosomal damage, and the numbers of exchange aberrations were not significantly different between radiofrequency irradiation and sham-exposed cells. Similarly, the percentages of binucleated cells, the incidence of binucleated cells with one, two or three micronuclei, and the total numbers of micronuclei were not significantly different.

Study character:

Study funded by

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