Aim of the study was to investigate the extent of possible genetic damage caused by radiofrequency irradiation (835.62 MHz, 24 h, FDMA) on human blood lymphocytes in vitro.
|Exposure duration||continuous for 24 h|
|Chamber||The facility housed nine RTLs (radial transmission lines) mounted on two separate adjacent sliding shelves. The facility and RTLs were shielded to avoid interference. Each RTL was formed by two parallel horizontal metal plates 4.3 cm apart, with a lightweight composite top plate hinged to the bottom aluminium plate (0.6 cm thick) providing mechanical support and homogeneity of the flasks' temperatures. The waves travelling from the central conical antenna were terminated by an annulus of MW-absorbing foam backed by perforated aluminium. The total radius was 53.3 cm.|
|Setup||Each RTL was fully loaded with 16 T-75 culture flasks at a radius of 29.2 cm each containing 40 ml of liquid medium (about 5 mm depth) (two of them diluted blood). The distance of the cells settled at the bottom of the flask from the antenna was between 24.7 cm and 33.8 cm.|
|Additional info||Aliquots of blood that were sham-exposed or exposed to 1.5 Gy of γ radiation were used as negative or positive controls.|
The overall cytogenetic responses of the lymphocytes were similar although the absolute values were slightly different. The mitotic indices, the indices of abnormal cells showing chromosomal damage, and the numbers of exchange aberrations were not significantly different between radiofrequency irradiation and sham-exposed cells. Similarly, the percentages of binucleated cells, the incidence of binucleated cells with one, two or three micronuclei, and the total numbers of micronuclei were not significantly different.