Study type: Medical/biological study (experimental study)

Radiofrequency electromagnetic fields do not alter the cell cycle progression of C3H 10T and U87MG cells. med./bio.

Published in: Radiat Res 2001; 156 (6): 786-795

Aim of study (acc. to author)

To determine the effects of radiofrequency electromagnetic fields (RF EMFs) exposure on cell cycle progression in vitro by the method of flow cytometric bromodeoxyuridine pulse-chase.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 835.62 MHz
Modulation type: FM, CW
Exposure duration: continuous for up to 13 h or up to 100 h
  • SAR: 0.6 W/kg spatial average (± 0.3 W/kg)
Exposure 2: 847.74 MHz
Exposure duration: continuous for up to 13 h or up to 100 h
  • SAR: 0.6 W/kg spatial average (± 0.3 W/kg)

Exposure 1

Main characteristics
Frequency 835.62 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for up to 13 h or up to 100 h
Modulation
Modulation type FM, CW
Additional info

FMCW

Exposure setup
Exposure source
Chamber The radial transmission line (RTL) exposure system comprised nine RTL chambers (three each for sham, FMCW and CDMA) the temperature of which was maintained at 37.0 ± 0.3°C.
Setup The RTL was fully loaded with sixteen 75-cm² tissue culture flasks, each containing 40 ml of culture medium. For each flask removed for cell harvesting, a dummy flask was inserted.
Sham exposure A sham exposure was conducted.
Additional info Short-term exposures were performed for up to 13 h. Long-term exposures were performed for up to 100 h with flasks being removed at 24, 48, 72, and 96 h.
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.6 W/kg spatial average measured - ± 0.3 W/kg

Exposure 2

Main characteristics
Frequency 847.74 MHz
Type
Charakteristic
  • guided field
Exposure duration continuous for up to 13 h or up to 100 h
Modulation
Modulation type cf. additional info
Additional info

CDMA

Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.6 W/kg spatial average measured - ± 0.3 W/kg

Reference articles

  • Goswami PC et al. (1999): Proto-oncogene mRNA levels and activities of multiple transcription factors in C3H 10T 1/2 murine embryonic fibroblasts exposed to 835.62 and 847.74 MHz cellular phone communication frequency radiation.
  • Moros EG et al. (1999): The radial transmission line as a broad-band shielded exposure system for microwave irradiation of large numbers of culture flasks.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No changes in the cell cycle parameters were observed. At frequencies and power tested, radiofrequency electromagnetic fields exposure has no effect on cell cycle progression in vitro.

Study character:

Study funded by

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