Medical/biological study (experimental study)

One week of exposure to 50 Hz, vertical magnetic field does not reduce urinary 6-sulphatoxymelatonin excretion of male wistar rats med./bio.

By: Bakos J, Nagy N, Thuroczy G, Szabo LD

Published in: Bioelectromagnetics 2002; 23 (3): 245-248

Aim of study (acc. to author)

To investigate the effect of a magnetic field (MF) exposure on 6-sulphatoxymelatonin urinary excretion in rats.

Background/further details

6-sulphatoxymelatonin is the major metabolite of melatonin and could easily non-invasive determined in excreted urine.
12 animals were used in this study (divided in to two groups/6 animals each). The groups were exposed to 100 µT and 50 µT flux density, respectively. After 2 weeks of acclimatisation to the light/dark cycle (12:12 h, light from 6.00 pm to 6.00 am) nocturnal urine was collected ad the end of the dark phase for four weeks. Animals were exposed to the magnetic field in the second week daily from 8.00 am to 4.00 pm.

Endpoint

endocrine changes: amount of 6-sulphatoxymelatonin in excreted urine

Exposure

- 50/60 Hz
- magnetic field

Exposure	Parameters
Exposure 1: 50 Hz Modulation type: CW Exposure duration: 8 h/day, for 1 week	magnetic flux density: 50 µT minimum magnetic flux density: 100 µT maximum

Exposure 1

Main characteristics

Frequency	50 Hz
Type	magnetic field
Waveform	sinusoidal
Polarization	linear
Exposure duration	8 h/day, for 1 week
Additional info	vertical

Modulation

Modulation type	CW

Exposure setup

Exposure source	not specified Mel-00011
Chamber	plastic box 40x30x10cm

Parameters

Measurand	Value	Type	Method	Mass	Remarks
magnetic flux density	50 µT	minimum	measured	-	-
magnetic flux density	100 µT	maximum	measured	-	-

Additional parameter details

earth MF 48µT, background at 50 Hz 0,1-0,3 µT

Reference articles

Bakos J et al. (1995): Sinusoidal 50 Hz, 500 microT magnetic field has no acute effect on urinary 6-sulphatoxymelatonin in Wistar rats

Exposed system:

animal
rat/Wistar
whole body

Methods Endpoint/measurement parameters/methodology

endocrine changes: amount of 6-sulphatoxymelatonin in excreted urine (radioimmunoassay, normalized to urinary creatinine)

Investigated system:

isolated bio./chem. substance
6-sulphatoxymelatonin
investigation on living organism

Investigated organ system:

endocrine system

Time of investigation:

before exposure
during exposure
after exposure

Main outcome of study (acc. to author)

The excretion of the primary metabolite of melatonin in the urine, 6-sulphatoxymelatonin, was not statistically significant changed during or after magnetic field exposure.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Hungarian Ministry of Welfare

Seifpanahi-Shabani H et al. (2016): Long-term Exposure to Extremely Low Frequency Electromagnetic Field and Melatonin Production by Blood Cells
Dyche J et al. (2012): Effects of power frequency electromagnetic fields on melatonin and sleep in the rat
Davis S et al. (2006): Effects of 60-Hz magnetic field exposure on nocturnal 6-sulfatoxymelatonin, estrogens, luteinizing hormone, and follicle-stimulating hormone in healthy reproductive-age women: results of a crossover trial
Gobba F et al. (2006): No association between occupational exposure to ELF magnetic field and urinary 6-sulfatoximelatonin in workers
Youngstedt SD et al. (2002): No association of 6-sulfatoxymelatonin with in-bed 60-Hz magnetic field exposure or illumination level among older adults
Noonan CW et al. (2002): Relationship between amyloid beta protein and melatonin metabolite in a study of electric utility workers
Chacon L (2000): 50-Hz Sinusoidal Magnetic Field Effect On In Vitro Pineal N-Acetyltransferase Activity
Heikkinen P et al. (1999): Chronic exposure to 50-Hz magnetic fields or 900-MHz electromagnetic fields does not alter nocturnal 6-hydroxymelatonin sulfate secretion in CBA/S mice
Selmaoui B et al. (1999): Age-related differences in serum melatonin and pineal NAT activity and in the response of rat pineal to a 50-Hz magnetic field
Bakos J et al. (1999): Urinary 6-Sulphatoxymelatonin Excretion of Rats is not Changed by 24 Hours of Exposure to A Horizontal 50-Hz, 100-μT Magnetic Field
Bakos J et al. (1997): Urinary 6-sulphatoxymelatonin excretion is increased in rats after 24 hours of exposure to vertical 50 Hz, 100 microT magnetic field
Bakos J et al. (1995): Sinusoidal 50 Hz, 500 microT magnetic field has no acute effect on urinary 6-sulphatoxymelatonin in Wistar rats