この研究は、マウス海馬スライスから記録される誘発電位（集合スパイク）に対する低い繰返し率のパルス磁界（LRMF）の影響を調べた。LRMFは2つのプロトコルで与えた。プロトコルAは、一定の強度（15 mT）で周波数0.03〜0.5 Hzの範囲のLRMFを与えた。この場合の結果は、LRMFにより誘発電位が増幅された；0.16 Hzの繰返し周波数が最も効果的であり、集合スパイクを280 %以上増加させた；その一方、スライスの50 %において、自発活動、発作、および神経活動の停止も増加した。プロトコルBは、強度は可変（9-15 mT）で、5分から20分の範囲の異なる時間サイクルでLRMFを与えた。この場合の結果は、プロトコルBのLRMFばく露を受けたすべてのスライスで、集合スパイクの振幅の増加が観察された；最長のばく露が最も効果的であった；発作もなく、自発活動の増加も観察されなかった。これらの結果は、著者らによる以前のデータを支持および拡張し、印加磁界のパターンと神経系への影響の関係の特性をさらに明らかにした、と報告している。
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To investigate the effects of pulsed magnetic fields at a very low repetition rate on evoked potential in vitro from mouse hippocampal slices.
Two different experimental protocols were applied: In protocol A the magnetic field with a constant strength of 15 mT was used within a frequency range from 0.03 to 0.5 Hz. In protocol B a field of variable intensity from 9 to 15 mT and with different duration cycles from 5 to 10 minutes was used.
| ばく露 | パラメータ |
|---|---|
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ばく露1:
ばく露時間:
intermittent: 1, 3, 5, 7, and 15 s on/off, for 30 min
protocol A
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ばく露2:
ばく露時間:
intermittent, 20 s on/off, for a total of 10 min 40 s
protocol B, paradigm 1
|
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ばく露3:
ばく露時間:
intermittent, 5 s on/off, for a total of 6 min 30 s
protocol B, paradigm 2
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ばく露4:
ばく露時間:
intermittent, 2 s on/off, for a total of 3 min 15 s
protocol B, paradigm 3
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In experiments applying protocol B, the hippocampal slices were divided into three groups that were exposed to different combinations of three paradigms: all three paradigms successively, paradigm 1 followed immediately by paradigm 2, or paradigm 1 alone.
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | The cylindrical slice chamber was surrounded by a water-containing chamber inserted into a circular acrylic frame (19 cm in diameter) that was vertically streamed by a constant flow of cooling air and surrounded by four coils (96 turns) mounted perpendicularly to the plane of the slice. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 15 mT | - | 測定値 | - | - |
| ばく露の発生源／構造 |
|
|---|---|
| Additional information | The 9 mT and 12 mT cycles were repeated 4 times, and the 15 mT cycle was repeated 8 times. |
| ばく露の発生源／構造 |
|
|---|---|
| Additional information | The on/off cycle was repeated 9 times, followed by a 20 s break and 6 more repetitions. Another 220 s break followed before initiation of paradigm 3. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 15 mT | - | 測定値 | - | - |
| ばく露の発生源／構造 |
|
|---|---|
| Additional information | The on/off cycle was repeated 12, 4, 3, 5, and 6 times followed by 30, 10, 25, and 10 s breaks, respectively. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 15 mT | - | 測定値 | - | - |
A frequency-dependent amplification of the potentials was found whilst magnetic fields were applied according to protocol A. At this a frequency of 0.16 Hz was the most effective. An increase in the magnitude of the spikes were found caused by magnetic field exposure according to protocol B.
The results of this study demonstrate the influence of the applied magnetic fields on the nervous system.
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