研究のタイプ: 医学/生物学の研究 (experimental study)

[直流磁界ばく露時のhepG2細胞の時間依存性変調] med./bio.

Time dependent modifications of Hep G2 cells during exposure to static magnetic fields.

掲載誌: Bioelectromagnetics 2005; 26 (4): 275-286

この研究は、6 mTの静磁界への24時間のばく露中のHep G2細胞の形態変化、すなわち、細胞形状、細胞表面糖残基、細胞骨格、およびアポトーシス、を光学顕微鏡法電子顕微鏡法および細胞化学によって調べた。静磁界ばく露の全期間中、細胞の形状および表面の変化の進行を観察した。その結果、対照細胞は、細胞表面が短い微絨毛で覆われた多面体であったが、ばく露細胞は細長くなり、細胞表面には不整な微絨毛がランダムに分布していた;ばく露期間の終了時、細胞培養皿から一部は剥離して平坦でない形状であった;しかし、ばく露期間を通して、オルガネラの形態は変化せず、細胞増殖部分的にしか影響を受けなかった;細胞形状の変化と並行して、微小フィラメント、微小管、および細胞表面のConA-FITCおよびRicinus communnis(トウゴマ)-FITC標識部位の量と分布も時間に依存して変化した;ばく露開始時にはほとんど無視できたアポトーシスは、24時間の連続ばく露後に約20%に増加した、と報告している。

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研究目的(著者による)

To study morphological modifications, i.e. cell shape, cell surface sugar residues, cytoskeleton, and apoptosis of Hep G2 cells (a hepatic transformed cell line) during 24 h exposure to 6 mT static magnetic field.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
  • DC/static
ばく露時間: continuous for 24 h

ばく露1

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 continuous for 24 h
Additional information Static magnetic field
ばく露装置
ばく露の発生源/構造
  • Neodymium magnetic disks, 10 mm in diameter x 5 mm high
ばく露装置の詳細 Magnetic disks were placed under the Perti dishes
Additional information Control experiments were carried out simultaneously withourt the magnetic disks.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 6 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The results indicate that 6 mT static magnetic field exposure exerts time dependent biological effects on Hep G2 cells.
Progressive modifications of cell shape and surface were found during the entire period of exposure. Exposed cells were elongated with many irregular microvilli randomly distributed on the cell surface.
At the end of the irradiation period, the cells had a less flat shape due to partial detachment from the culture dishes. However, throughout the period of irradiation, the morphology of the organelles remained unmodified and cell proliferation was only partially affected. In parallel with cell shape changes, the microfilaments and microtubules, as well as the quantity and distribution of surface Ricinus communnis-FITC and ConA-FITC labeling sites, were modified in a time dependant manner.
Apoptosis, which was almost negligible at the beginning of experiment, increased to about 20% after 24 h of continuous exposure. The induction of apoptosis was likely due to the increment of intracellular calcium concentration during exposure.

研究の種別:

研究助成