研究のタイプ: 医学/生物学の研究 (experimental study)

[培養されたネズミの海馬ニューロンのN-メチル-D-アスパラギン酸塩レセプターチャンネルのブロックに対する連日反復的直流磁界ばく露への反作用] med./bio.

Counteraction by repetitive daily exposure to static magnetism against sustained blockade of N-methyl-D-aspartate receptor channels in cultured rat hippocampal neurons.

掲載誌: J Neurosci Res 2005; 80 (4): 491-500

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研究目的(著者による)

To evaluate possible protective properties of repetitive daily exposure to static magnetic fields against the neurotoxicity of sustained blockade of N-methyl-D-aspartate (NMDA) receptor channels by dizocilpine (MK-801) in cultured rat hippocampal neurons.

詳細情報

The NMDA receptor antagonist MK-801 was added into culture medium at a concentration of 1-100 µM in hippocampal neurons cultured for up to 8 days in vitro.
Sustained exposure to static magnetic fields modulates cellular maturation and development, at least in part through the up-regulation of NMDA receptor channels.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
  • DC/static
ばく露時間: repeated daily exposure, 15 min/day for 8 consecutive days
ばく露2:
  • DC/static
ばく露時間: continuous for 3 days

ばく露1

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 repeated daily exposure, 15 min/day for 8 consecutive days
ばく露装置
ばく露の発生源/構造
  • permanent ferrite magnets, 10 x 15 x 2.5 cm
ばく露装置の詳細 ferrite magnets with N pole upwards were placed on both sides of the culture dishes which were located at the center of the magnetic fields at a distance of 10 cm from each other. Control dishes were treated under the same environmental conditions as for the exposure groups in the absence of ferrite magnets.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 100 mT - 測定値 - -

ばく露2

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 continuous for 3 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 100 mT - 測定値 - -

Reference articles

  • Hirai T et al. (2004): [静的磁界持続ばく露後の未熟培養ラット海馬神経細胞における機能的変化]

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In hippocampal neuronal cultures treated with MK-801 a significant decrease was found in the expression of MAP2 (a major component of cross-bridges between microtubules in dendrites) as well as mRNA for both BDNF and GAP43 (a marker of neuronal maturity that is associated with neuronal development, axonal regeneration, and synaptogenesis). Additionally, a decreased viability was observed. MK-801 not only decreased the expression of the NR1 subunit of NMDA receptors, but also increased NR2A expression, without affecting NR2B expression.
Repetitive daily exposure to static magnetic fields led to a decrease in the expression of MAP2 (to approximately 50% of the control), without significantly affecting cell viability or the expression of neuronal nuclei and GAP43.
However, the exposure to repetitive magnetism prevented decreases in both BDNF mRNA and MAP2. In addition it increased the expression of NR2A subunit, without altering NR1 expression in neurons cultured in the presence of MK-801.
These data suggest that repetitive magnetism may at least in part counteract the neurotoxicity of MK-801 through modulation of the expression of particular NMDA receptor subunits in cultured rat hippocampal neurons.

研究の種別:

研究助成

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