研究のタイプ: 医学/生物学の研究 (experimental study)

[培養褐色細胞腫細胞におけるサイトゾルCa2 +によるCa2 +放出の一過性上昇に対する時間変動する強磁界の影響] med./bio.

Effects of a time-varying strong magnetic field on transient increase in Ca(2+) release induced by cytosolic Ca(2+) in cultured pheochromocytoma cells.

掲載誌: Biochimica et Biophysica Acta - General Subjects 2005; 1724 (1-2): 8-16

この研究は、褐色細胞腫PC 12)細胞に時間変化する1.51 T磁界へのばく露を与え、細胞内Ca(2+)濃度([Ca(2 +)] i)の変化を調べた。その結果、磁界ばく露は、Ca(2+)なしの培地カフェインを添加することによって誘導される細胞内Ca(2+)濃度([Ca(2 +)] i)の上昇を阻害した;この阻害は15分間のばく露後に発生し、少なくとも2時間維持された;サイクリックADPリボースを内蔵した細胞で、[Ca(2 +)] iは急激に上昇したが、この上昇を2時間のばく露は有意に阻害した;ATPの添加は、IP(3)受容体を介した細胞内Ca(2+)放出の一時的な増加を誘発したが、この増加はばく露により強く阻害された;これらの知見は、磁界ばく露がPC 12細胞におけるIP(3)およびリアノジン受容体の両方によって媒介されるCa(2+)放出を強く阻害することを示した、と報告している。

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研究目的(著者による)

To study the effects of exposure to a time-varying 1.5 T magnetic field on Ca2+ release from intracellular Ca2+ stores mediated by ryanodine and IP3 (inositol trisphosphate) receptors in cells.

詳細情報

After assaying fluorescence in Ca2+-free medium, cells were treated with any one of the drugs such as caffeine, ATP and thapsigargin to induce a transient change in intracellular calcium concentration.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
Modulation type: pulsed
ばく露時間: intermittent for 15 min, 30 min, 1 h or 2 hr; 3 s on/off cycle
  • 磁束密度: 1.51 T maximum
  • 電流密度: 28 mA/m² mean (11 mA/m² min value; induced eddy current densities in the medium)

ばく露1

主たる特性
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 intermittent for 15 min, 30 min, 1 h or 2 hr; 3 s on/off cycle
Modulation
Modulation type pulsed
ばく露装置
ばく露の発生源/構造
  • electromagnet
チャンバの詳細 Incubator (129 mm in diameter, 20 mm thick) containing 4 culture dishes and maintained at 37°C
ばく露装置の詳細 incubator placed in the gap between the two poles of the electromagnet
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.51 T maximum 指定なし - -
電流密度 28 mA/m² mean 推定値 - 11 mA/m² min value; induced eddy current densities in the medium

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Exposure of PC-12 cells to a time-varying 1.51 T magnetic field inhibited an increase in the intracellular Ca2+ concentration induced by addition of caffeine to Ca2+-free medium. This inhibition occurred after a 15 min exposure and was maintained for at least two hours.
The intracellular Ca2+ concentration increased in cells loaded with cyclic ADP-ribose (a ryanodine receptor stimulator), and two hours exposure significantly suppressed the increase. Addition of ATP induced a transient increase in intracellular Ca2+ release mediated by inositol trisphosphate receptor. This increase was strongly inhibited by the exposure.
The data indicated that the magnetic field exposure strongly inhibited Ca2+ release mediated by both inositol trisphosphate and ryanodine receptors. However, thapsigargin-induced Ca2+ influx across the cell membrane was unaffected.
The ATP concentration was maintained at the normal level during the 2 h exposure, indicating that ATP hydrolysis was unchanged.

研究の種別:

研究助成

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