研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒト好中球細胞株のカルシウムシグナル伝達は低周波電磁界に影響されない] med./bio.

Calcium signalling in human neutrophil cell lines is not affected by low-frequency electromagnetic fields.

掲載誌: Bioelectromagnetics 2015; 36 (6): 430-443

この研究は、ヒト好中球細胞株(HL-60、PLB-985)のカルシウムシグナル伝達に対する低周波電磁界LF EMFs:Immunent波形または正弦波磁束密度5μT、300μT、500μT)の影響の可能性を調べた。好中性顆粒球では、受容体に結合したGタンパク質の活性化により、特定のカルシウムチャネルを介したカルシウム流出または流入が生じる。この現象へのLF EMFsの関与を実験で調べた。その結果、走化性刺激時のカルシウム動員は、30分間の短期ばく露または長期ばく露により変化しなかった;波形磁束密度を変えても結果は変わらなかった、と報告している。

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研究目的(著者による)

The adverse effects of exposure of human neutrophil granulocyte-like cells to a 50 Hz magnetic field or to a combination of several extremely low frequency and intermediate frequency fields on calcium signal pathways should be investigated.

詳細情報

In two different cell lines, HL-60 and PLB-985 cells, a differentiation into neutrophil-like cells was induced by the addition of DMSO. The combination of different low-frequency and intermediate frequency fields was called "Immunent field".
Both cell lines were exposed to two different types of fields (50 Hz and Immunent field) and 4 different magnetic flux densities (2,5 mT, 500 µT, 300 µT, and 5 µT) for 3 minutes, 30 minutes, 4 days or 5 days.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 30 minutes
ばく露2: 50 Hz–2.6 kHz
ばく露時間: continuous for 30 minutes
Immunent field
ばく露3: 50 Hz
ばく露時間: continuous for 30 minutes, 4 days or 5 days
ばく露4: 50 Hz–2.6 kHz
ばく露時間: continuous for 30 minutes, 4 days or 5 days
Immunent field
ばく露5: 50 Hz
ばく露時間: intermittent for a total of up to 3 minutes
for flow cytometry

General information

background alternating current magnetic flux densities at the place of exposure and sham exposure were less than 0.2 µT and ambient direct current static magnetic fields were 10 µT (except for exposure 5)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 30 minutes
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cell culture flasks
ばく露装置の詳細 coil was placed inside a standard cell culture incubator with 37°C ± 0.2°C and 5% CO2; cell culture flasks were placed in the central region of the coils, perpendicular to vertical magnetic field lines; copper wire coil was wound on a double co-axial cylinder with an inner (65 turns; Ø 184 mm) and outer cylinder (2 times 8 turns; Ø 400 mm) made of polymethyl methacrylate; inhomogeneity within exposure area was < 0.4%
Sham exposure A sham exposure was conducted.
Additional information two identical cell culture incubators with identical exposure coil inside were used and randomly assigned to be active or sham for each experiment
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 µT - 測定値 - -
電界強度 1.57 mV/m maximum - - -

ばく露2

主たる特性
周波数 50 Hz–2.6 kHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 30 minutes
Additional information Immunent field
Additional information combination of four block waves at frequencies of 320, 730, 880 and 2600 Hz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 µT - 測定値 - -
電界強度 0.3 V/m maximum - - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 30 minutes, 4 days or 5 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 500 µT - 測定値 - -
電界強度 1.57 mV/m maximum - - -

ばく露4

主たる特性
周波数 50 Hz–2.6 kHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 30 minutes, 4 days or 5 days
Additional information Immunent field
Additional information combination of four block waves at frequencies of 320, 730, 880 and 2600 Hz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 300 µT - 測定値 - -
電界強度 0.3 V/m maximum - - -

ばく露5

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 intermittent for a total of up to 3 minutes
Additional information for flow cytometry
ばく露装置
ばく露の発生源/構造
チャンバの詳細 test tubes
ばく露装置の詳細 custom-made exposure system fitting around the test tube inside the flow cytometer; the circular coil consisted of 0.5 mm tightly wound copper wire around a polyether ether ketone body with an inner diameter of 38 mm (100 turns); the direction of the field was parallel to the height of the test tube
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2.5 mT - 測定値 - -
電界強度 1.57 mV/m maximum - - -

Reference articles

  • Bouwens M et al. (2012): [低周波電磁界はヒト単球及び免疫細胞株における炎症性遺伝子及びタンパク質の応答を変化させない]
  • de Kleijn S et al. (2011): [超低周波電磁界ばく露はヒト末梢血単核細胞におけるToll様受容体シグナル伝達を変化させない]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

No significant differences could be found between the exposure groups and their corresponding sham exposure groups.
The authors conclude that exposure of human neutrophil granulocyte-like cells to a 50 Hz magnetic field or to a combination of several extremely low frequency and intermediate frequency fields has no effect on calcium signal pathways.

研究の種別:

研究助成

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