研究のタイプ: 医学/生物学の研究 (experimental study)

[ラットでの後肢の痛みを伴う電気刺激および皮膚のEHF照射の後における視床下部細胞でのナチュラルキラー細胞の細胞傷害活性およびc-Fosタンパク質合成] med./bio.

Natural killer cell cytotoxic activity and c-Fos protein synthesis in rat hypothalamic cells after painful electric stimulation of the hind limbs and EHF irradiation of the skin.

掲載誌: Med Sci Monit 2005; 11 (9): BR309-BR315

この研究は、痛みを伴う電気刺激で生じるナチュラルキラー(NK)細胞活性の変化および視床下部構造でのc-Fos陽性細胞数の変化を測定し、次に、皮膚への超高周波(EHF)照射により、このような変化のプロセスが調節されるかを調べた。ウィスターラットの後肢に痛みを伴う電気刺激を与えるとともに、皮膚にEHF照射を組み合わせる実験を行なった。脾臓NK細胞の細胞毒性活性は、インビトロでのK-562腫瘍細胞溶解能力により評価した。c-Fos様タンパク質は、免疫ペルオキシダーゼ法で検出した。その結果、痛みを伴う電気刺激は、脾臓NK細胞の細胞毒性有意な低下、および視床下部構造(特に視床下部前核(AHN)および視床下部外側野(LHA))におけるc-Fos陽性細胞数の劇的な増加と関連した;電気刺激の前後に1回ずつ2回のEHFばく露を行うと、脾臓NK細胞活性の抑制が妨げられ、視床下部腹内側核(VMH)および基底LHAに発現するc-Fos陽性細胞の数が減少した;AHNおよびLHAのc-Fos陽性細胞数とNK細胞の細胞毒性活性の間には負の相関が見られた、と報告している。

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研究目的(著者による)

To assess changes in natural killer cell activity and the number of c-Fos-positive cells in hypothalamic structures induced by painful electrical stimulation and to use extremely high frequency irradiation of the skin to modulate these processes.

詳細情報

Experiments were performed on rats subjected to painful electrical stimulation of the hind limbs combined with extremely high frequency irradiation of the skin.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 42.25 GHz
ばく露時間: continuous, twice 40 min exposures with 40 min pause between them

General information

The stress model was based on electrical pain stimulation which was carried out by applying 2.5 mA shocks of 1 s duration with a mean of 10 impulses/ min for a period of 40 min by clasping the electrodes tightly to the rats ankles. The pain stimulation was given either before or after the exposure or applied alone.

ばく露1

主たる特性
周波数 42.25 GHz
特性
  • guided field
ばく露時間 continuous, twice 40 min exposures with 40 min pause between them
Modulation
Modulation type unspecified
ばく露装置
ばく露の発生源/構造
  • 導波管
  • G4-141 electromagnetic field generator
ばく露装置の詳細 Rats placed into a cylindrical plastic container; their skin was exposed in the following three places: both shins (hind legs) 3 mm below and 3 mm lateral to the center of the knee and the back of the neck between the seventh cervical and the first thoracic vertebrae along the mid line.
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力 20 mW unspecified 指定なし - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Painful electric stimulation was associated with a significant decrease in splenic natural killer cell cytotoxicity and a dramatic increase in c-Fos-positive cell counts in some hypothalamic structures. Two irradiations, one before and one after electric stimulation, prevented the decrease of natural killer cell activity and caused a reduction in the number of c-Fos-positive cells expressed in one hypothalamic nucleus and a specific hypothalamic area. Negative correlation was found between c-Fos-positive cell counts in one hypothalamic nucleus/a specific hypothalamic area and the cytotoxic activity of natural killer cells.
The data suggest that painful electric stimulation of the hind limbs of rats causes a reorganization of the central mechanism regulating splenic natural killer cell activity resulting in a decrease in their cytotoxicity, and that extremely high frequency exposure of the skin prevents this reorganization, thus protecting splenic natural killer cell activity from the impairment induced by the painful electric stimulation.

研究の種別:

研究助成