研究のタイプ: 医学/生物学の研究 (experimental study)

[マウスm5S細胞での染色体異常に対する広範囲のSARの無線周波電磁界の影響] med./bio.

Effect of high-frequency electromagnetic fields with a wide range of SARs on chromosomal aberrations in murine m5S cells.

掲載誌: Mutat Res Genet Toxicol Environ Mutagen 2005; 587 (1-2): 114-119

この研究は、マウスm5S細胞を用いて、2.45 GHzの高周波電磁界(HFEMF)へのばく露後の染色体異常誘導の有無を調べた。ばく露レベルは、連続波(CW)の場合、平均SARは5、10、20、50、100 W/ kg、パルス波(PW)の場合、SARの平均値は100 W / kg(最大900 W / kg)とした。ばく露時間はいずれも2時間である。擬似ばく露群を対照とし、陽性対照にはマイトマイシンC(MMC)処置群またはX線照射群を用いた。全ての群について、染色体の構造、染色分体型および染色体型の変化を調べた。その結果、 SAR = 5 - 100 W/ kg のCWおよび平均SAR = 100 W/ kg のPWのHFEMFばく露群において、擬似ばく露対照群と比較して有意差は観察されなかった;MMCおよびX線の処置群では、染色分体型および染色体型の異常発生率が増加した、と報告している。

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研究目的(著者による)

To study the induction of chromosomal aberrations in mouse cells after exposure to high frequency electromagnetic fields at 2.45 GHz with a wide range of SAR-values.

詳細情報

Mitomycin C (0.1 µg/ml) and x-ray (3 Gy) treatment were used as positive controls.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: CW
ばく露時間: continuous for 2 h
  • SAR: 100 W/kg maximum (50 W/kg, 20 W/kg, 10 W/kg and 5 W/kg)
ばく露2: 2.45 GHz
Modulation type: pulsed
ばく露時間: intermittent for 2 h, 1 s field on/8 or 17 s off
  • SAR: 50 W/kg mean (900 W/kg corresponding peak value applied for 1 s followed by 17 s pause resulting in 50 W/kg mean value)
  • SAR: 100 W/kg mean (900 W/kg corresponding peak value applied for 1 s followed by 8 s pause resulting in 100 W/kg mean value)

ばく露1

主たる特性
周波数 2.45 GHz
特性
  • guided field
ばく露時間 continuous for 2 h
Additional information Reference article: S.Koyama, Y.Isozumi, Y.Suzuki, M.Taki, J.Miyakoshi. Effects of 2.45 GHz electromagnetic fields with a wide range of SARs on micronucleus formation in CHO-K1 cells, Scientific World Journal 4 (2004) 29-40.
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
チャンバの詳細 One end of the waveguide was terminated with short circuit plate to generate standing waves.
ばく露装置の詳細 Sham exposure was conducted in the same manner as the RF exposure but in the absence of RF field. Positive control cells were treated with 3 Gy of X-ray or mitomycin C (0.1 µg/ml) for 2 h.
パラメータ
測定量 種別 Method Mass 備考
SAR 100 W/kg maximum 指定なし - 50 W/kg, 20 W/kg, 10 W/kg and 5 W/kg

ばく露2

主たる特性
周波数 2.45 GHz
特性
  • guided field
ばく露時間 intermittent for 2 h, 1 s field on/8 or 17 s off
Modulation
Modulation type pulsed
Pulse width 1 s
Additional information

30 s cycle

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 50 W/kg mean 指定なし - 900 W/kg corresponding peak value applied for 1 s followed by 17 s pause resulting in 50 W/kg mean value
SAR 100 W/kg mean 指定なし - 900 W/kg corresponding peak value applied for 1 s followed by 8 s pause resulting in 100 W/kg mean value

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

No significant differences were revealed following exposure to high frequency electromagnetic fields at SAR values from 5 to 100 W/kg (continuous wave) and at a mean SAR of 100 W/kg (pulsed wave, a maximum SAR of 900 W/kg) compared with sham-exposed controls.
Treatments with mitomycin C and X-rays increased the frequency of chromatid-type and chromosome-type aberrations.
In conclusion, high frequency electromagnetic field exposures at 2.45 GHz for two hours with up to 100 W/kg SAR (continuous wave) and an average SAR of 100 W/kg (pulsed wave, a maximum SAR of 900 W/kg) do not induce chromosome aberrations in these mouse cells. Furthermore, there was no difference between exposures to continuous wave and pulsed wave high frequency electromagnetic fields.

研究の種別:

研究助成

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