研究のタイプ: 医学/生物学の研究 (experimental study)

[内皮細胞におけるトロンビンで刺激されたエンドセリン-1の生成を磁界は抑制する] med./bio.

Electromagnetic fields inhibit endothelin-1 production stimulated by thrombin in endothelial cells.

掲載誌: J Int Med Res 2005; 33 (5): 545-554

この研究は、培養内皮細胞電磁界EMF)のばく露を与え、それによって強力な血管収縮剤であるエンドセリン-1(ET-1)の産生が変化するか否かを調べた。実験にはいくつかの種類の培養内皮細胞を用いた。結果として、ヒトの臍静脈および微小血管の内皮細胞においては、EMFばく露によりET-1の基礎レベルが低下した;一方、ウシおよびヒトの大動脈内皮細胞においては、ET-1の基礎レベルはEMFばく露によって変化しなかった;4種類すべての内皮細胞のトロンビン刺激性ET-1産生は、EMFばく露量に依存して有意に抑制された;また、4種類すべての内皮細胞において、トロンビン誘発エンドセリン1 mRNA発現がEMFばく露により有意に抑制された;ET-1生産に対するEMFばく露による抑制効果は、一酸化窒素合成酵素阻害剤NG-モノメチル-L-アルギニン(10(-3)mol / 1)により消失した、と報告している。

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研究目的(著者による)

To study the effect of radiofrequency electromagnetic field on basal and thrombin-stimulated endothelin-1 (a potent vasoconstrictor) expression and production in cultured cells from a variety of sources (bovine aortic endothelial cells, human umbilical vascular endothelial cells, human aortic endothelial cells, and human microvascular endothelial cells).

詳細情報

Electromagnetic field exposure has been found to induce arteriolar dilatation, but the mechanism of action remains largely unknown.
To study whether nitric oxide may participate in the mediation of endothelin-1 production by electromagnetic field, a competitive inhibitor of nitric oxide synthase (NG-monomethyl-L-arginine) was used to block the synthesis of nitric oxide in the cell cultures.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 10 MHz
Modulation type: pulsed
ばく露時間: continuous for 8 h or 24 h
ばく露2: 10 MHz
Modulation type: pulsed
ばく露時間: continuous for 8 h or 24 h
ばく露3: 10 MHz
Modulation type: pulsed
ばく露時間: continuous for 8 h or 24 h

ばく露1

主たる特性
周波数 10 MHz
ばく露時間 continuous for 8 h or 24 h
Modulation
Modulation type pulsed
Duty cycle 50 %
Repetition frequency 10,000 Hz
ばく露装置
ばく露の発生源/構造
  • coil plate consisting of circular loop generator coils
ばく露装置の詳細 96 well culture plates containing endothelial cells were placed on a coil plate. Cells were sham exposed for the same duration under the same set-up conditions.
Additional information The distance between the generator coil and the cells was 2.0 mm.
パラメータ
測定量 種別 Method Mass 備考
電界強度 1.25 V/m unspecified 校正 - -
SAR 0.98 mW/kg unspecified 指定なし - -

ばく露2

主たる特性
周波数 10 MHz
ばく露時間 continuous for 8 h or 24 h
Modulation
Modulation type pulsed
Duty cycle 50 %
Repetition frequency 10,000 Hz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 2.31 mW/kg unspecified 指定なし - -
電界強度 1.92 V/m unspecified 校正 - -

ばく露3

主たる特性
周波数 10 MHz
ばく露時間 continuous for 8 h or 24 h
Modulation
Modulation type pulsed
Duty cycle 50 %
Repetition frequency 10,000 Hz
ばく露装置
ばく露の発生源/構造
  • coil plate consisting of circular loop generator coils (different diameter)
ばく露装置の詳細 100 mm culture dish containing endothelial cells was placed on a coil plate. Cells were sham exposed for the same duration under the same set-up conditions.
Additional information The distance between the generator coil and the cells was 2.3 mm.
パラメータ
測定量 種別 Method Mass 備考
SAR 8.06 mW/kg unspecified 指定なし - -
電界強度 3.59 V/m unspecified 校正 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Electromagnetic field exposure reduced endothelin-1 basal levels in human umbilical vein and microvascular endothelial cells, but failed to reduce endothelin-1 basal levels in bovine and human aortic endothelial cells.
Electromagnetic field exposure significantly inhibited thrombin-stimulated (thrombin up-regulates the endothelin-1 production) endothelin-1 production in all four endothelial cell types in a dose-dependent manner.
Electromagnetic field irradiation significantly inhibited thrombin-induced endothelin-1 mRNA expression in all four cell types.
The inhibitory effect on endothelin-1 production was abolished by the nitric oxide synthase inhibitor.
These findings demonstrate that electromagnetic field exposure modulates endothelin-1 production in cultured vascular endothelial cells and the inhibitory effect is, at least partly, mediated through a nitric oxide-related pathway.

研究の種別:

研究助成