研究のタイプ: 医学／生物学の研究 (experimental study)

[CD95 + Tヘルパーヒトリンパ球に対するイン・ビトロでの無線周波数ばく露（携帯電話）の年齢依存的影響] med./bio.

Age-dependent effects of in vitro radiofrequency exposure (mobile phone) on CD95+ T helper human lymphocytes

著者: Capri M, Salvioli S, Altilia S, Sevini F, Remondini D, Mesirca P, Bersani F, Monti D, Franceschi C
掲載誌: Ann N Y Acad Sci 2006; 1067: 493-499
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この研究は、無線周波（RF）電磁界がヒトのリンパ球の活性化に影響を与える可能性を調べた。若年者および高齢者のドナーから採取された末梢血単核細胞（PBMC）に分裂促進刺激処置または非刺激処置を行い、それぞれにRF（1,800 MHz、SARは2 W / kg）のばく露または擬似ばく露を与えた。その後、非刺激および刺激されたCD4+ e CD8+ T細胞のCD25、CD95、CD28分子を分析した。その結果、どちらのドナー（若年者と高齢者）においても、RFばく露群と擬似ばく露群の間に、T細胞の亜群の割合の有意差はなかった；ただし、RFばく露後に、高齢者ドナーからの分裂促進刺激されたCD4+ Tリンパ球において、CD95発現のわずかではあるが有意な減少が観察された；この影響は若いドナーからのサンプルでは見られなかった；年齢に関連したこのような知見は、免疫応答調節におけるCD95分子の重要性を考えると注目に値する、と報告している。

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研究目的（著者による）

To clarify whether radiofrequency exposure at a SAR value comparable to that related to mobile phone irradiation is able to affect in vitro activation in human lymphocytes from 10 young and 8 elderly donors. Specifically CD25, CD95, and CD28 molecules on CD4+ helper and CD8+ cytotoxic T lymphocytes with and without mitogenic stimulation (anti-CD3 monoclonal antibody) were investigated.

詳細情報

Human T lymphocytes are affected by age and during age the two major subsets (CD4+ and CD8+ cells) change their ratio, their activity, and their phenotype.
The work is part of the REFLEX project (Risk Evaluation of Potential Environmental Hazards From Low Energy Electromagnetic Field Exposure Using Sensitive in vitro Methods), funded by the European Union.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: intermittent, 10 min on/20 min off for 44 h
  • SAR: 2 W/kg unspecified

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 intermittent, 10 min on/20 min off for 44 h
Additional information Reference article: Carpi, M., E.Scarcella, E.Bianchi, et al. 2004 b. 1800 MHz radiofrequency (mobile phones, different global system for mobile phone communication modulations) does not affect apoptosis and heat shock protein 70 level in peripheral blood mononuclear cells from young donors. Int.J.Radiat.Biol. 80: 389 - 397.
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

Talk modulated GSM signal
ばく露装置
ばく露の発生源／構造
チャンバの詳細 Two single mode resonant cavities placed in an incubator. One cavity was used for real exposure while other one was used for sham exposure.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg unspecified 指定なし - -

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

No significant changes in the percentage of these cell subsets were found between exposed and sham-exposed lymphocytes in both young and elderly donors.
Nevertheless, after radiofrequency exposure a slight, but significant, downregulation of CD95 expression in stimulated CD4+ T lymphocytes from elderly, but not from young donors was revealed. This age-related result is given the importance of a such molecule in regulation of the immune response. On the basis of such data the authors hypothesize that radiofrequency exposure could decrease the susceptibility to fas-induced apoptosis in elderly subjects (the fas gene encodes one of several proteins important to apoptosis).

研究の種別:

研究助成

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