研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒト好中球のインビトロでの走化性に対する900MHzRFの影響] med./bio.

Effects of 900-MHz Radio Frequencies on the Chemotaxis of Human Neutrophils in Vitro.

掲載誌: IEEE Trans Biomed Eng 2008; 55 (2): 795-797

【目的】ヒト好中球のcAMP濃度勾配にしたがって移動する能力(走化性)に対するRF電磁界の影響の研究。【方法】健常ドナーからの血液を、様々な温度および約0.4V/mの900MHz電磁界ばく露した。【結果】好中球の速度は、RFばく露なしの場合、35度から40度までは上昇し、40度でピークとなり、40度以上では低下することを観察した。900MHz電磁界ばく露を行った場合、同じ温度で観察された速度以上に上昇し、速度の最大値は種々の温度で観察された最大値をおよそ50%上回った。RFばく露がもたらす温度変化の計算値は1マイクロ度以下であった。動きの方向は、濃度勾配および電界方向に沿ったものから、濃度勾配に直角な方向および電界方向へと変化した。RF電磁界への好中球平均応答時間は約2.5分であった。

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研究目的(著者による)

To study the effects of radiofrequency fields on the ability of human neutrophils to follow concentration gradients of cyclic AMP (chemotaxis).

詳細情報

Cell's ability to move depends on the type of leukocyte and its age, the amount of anti-coagulant used, and other unknown variables in the condition of the donors. Blood was drawn of 8 students. The effect of different temperatures (22°C - 43°C) was also studied.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
ばく露時間: continuous for about 15 min

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for about 15 min
ばく露装置
ばく露の発生源/構造
  • microstrip transmission line, signal generator
チャンバの詳細 A drop of fluid containing neutrophils was covered with a cover slip to create a layer about 15 µm thick. The cover slip was sealed with Vaseline around the edges to reduce the effects of pressure, temperature, and vibration on the movement of the leukocyte cells and to prevent the sample from drying out.
ばく露装置の詳細 The slide was placed for about 15 min on the microscope stage heated to the desired temperature, thus allowing it to reach steady state.
Additional information The temperature was increased in a series of experiments by one degree from 22 °C to 43 °C both with and without RF exposure. The motion of the exposed cells was observed for about 15 min before and for about 15 min after applying the RF radiation.
パラメータ
測定量 種別 Method Mass 備考
電力 88.4 µW - 計算値 - -
電界強度 0.392 V/m - 計算値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The speed of the neutrophils increased with increasing temperatures from 35°C to 40°C where it peaked and then decreased above 40°C without radiofrequency exposure.
Under 900 MHz radiofrequency field exposure, the speed increased above the value observed at the same temperature, and the maximum speed exceeded the measured value at any temperature by approximately 50%. The calculated temperature change resulting from the radiofrequency exposure was less than one microdegree.
Upon radiofrequency exposure the neutrophils moved perpendicular to the direction along the cAMP gradient and to the applied electrical fields. Without radiofrequency these cells moved toward the cAMP stripe.
The authors conclude that the effects of radiofrequency exposure on neutrophil chemotaxis should be considered for further exploration in larger and more controlled studies.

研究の種別:

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