この研究は、低電力のMMW電磁放射が細胞生理学に及ぼす潜在的な影響を調べた。ヒトグリア細胞株U-251 MGに、電力密度0.14 mW / cm2の60.4 GHz電磁放射ばく露を与え、小胞体（ER）ストレスに対する潜在的影響の有無を評価した。ERは、環境からの刺激に非常に敏感で、その恒常性はさまざまな病状で変化することが知られている。また、ERストレスセンサであるBiP / GRP78の発現への影響をリアルタイムPCR法により調べた。その結果、60.4 GHzへのばく露は、ERストレスで見られるタンパク質の折りたたみ不全や分泌異常を引き起こさず、XBP1またはATF6の転写因子の成熟も誘導しないことが示された；またBiP / GRP78のmRNAレベルにもMMWばく露による大きな変化が見られないことが示された、と報告している。
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To study the potential effects of low-power millimeter wave exposure on cellular physiology (endoplasmic reticulum stress).
The frequency range of 60 GHz was considered in the context of their near-future applications in wireless communication systems. The endoplasmic reticulum is an organelle sensitive to a wide variety of environmental insults and involved in a number of pathologies and it is the site of synthesis and folding of secreted proteins. Perturbations of endoplasmic reticulum functions and the resulting induced pathway involves different transmembrane proteins or transcription factors (e.g. ATF6, XBP-1) which activate specific genes, such as the chaperone BiP/GRP78.
Separated transfection complexes were used: Cells were transfected with pSEAP (secreted alkaline phosphatase) reporter plasmid as marker for endoplasmic reticulum stress. This reporter enzyme is folded into its active conformation inside the endoplasmic reticulum, and only the correctly folded proteins are secreted into the medium.
Cells were transfected with BiP promoter-reporter plasmid to study stress-involved transcription factors XBP-1 and ATF6.
As endoplasmic reticulum-stress positive control, cells were treated for with thapsigargin or brefeldin or dimethyl sulfoxide.
|ばく露時間||continuous for 24 h, 48 h or 72 h|
Exposure to 60.4 GHz did not modify endoplasmic reticulum protein folding and secretion, nor induced XBP1 or ATF6 transcription factors maturation. There was no significant changes in gene expression of BiP/GRP78 and HSP70.
The data show that endoplasmic reticulum homeostasis does not undergo any modification at molecular level after exposure to low-power millimeter wave exposure at 60.4 GHz.