研究のタイプ: 医学/生物学の研究 (experimental study)

[マイクロ波照射による安定化後の脳酵素組織化学] tech./dosim.

Brain enzyme histochemistry following stabilization by microwave irradiation.

掲載誌: Histochem J 1988; 20 (6-7): 397-404

この研究は、脳ホモジネート中に存在する様々な酵素の活性に対するマイクロ波照射の影響を調べた。生化学アッセイの対象としたホモジネートは、(a)前処理なし、(b)生理食塩水中での標準的マイクロ波ばく露後のもの、(c)ホルマリン中での標準的マイクロ波ばく露後のもの、であった。マイクロ波-ホルマリン群では、全ての酵素活性が失われた。マイクロ波-生理食塩水群では、アルカリホスファターゼ、5'-ヌクレオチダーゼ、イソクエン酸およびコハク酸デヒドロゲナーゼの活性が低下した。対照的に、乳酸塩およびリンゴ酸デヒドロゲナーゼの活性は不変であり、アセチルコリンエステラーゼの活性は明らかに上昇した、と報告している。

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研究目的(acc. to editor)

Microwave techniques in conjunction with other fixation methods can be used in pathology and neuroanatomy for improved staining and histochemical investigations. This study compares several biochemical and histochemical assays concerning the investigation of a broad range of enzymes in microwaved rat brains.

詳細情報

The studied enzymes were: alkaline phosphatase, acetylcholinesterase, 5'-nucleotidase, catalase, cytochrome oxidase, isocitrat-, malat-, lactat- , and succinate dehydrogenase. Brain microwave exposure occured after perfusing the rats and removing the brains. After microwave treatment the brains were homogenized or microtome sectioned for biochemical or histochemical investigation respectively.
Two different perfusion fluids were used followed by microwave exposure: physiological saline and 10% formalin. Non-pretreated material poses as control.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: CW
ばく露時間: 30 min

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
ばく露時間 30 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • Mile domestic oven 696
チャンバの詳細 microwave was carried out in either saline or 10% formalin
パラメータ
測定量 種別 Method Mass 備考
電力 150 W - - - at 50°C

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Biochemical outcome: all enzyme activity was lost after formalin-microwave treatment. After saline-microwave treatment the activity of acetylcholinesterase was increased. The activities of malat- and lactat dehydrogenase were not altered while activites of alkaline phosphatase, 5'-nucleotidase, succinat- and isocitrat dehydrogenase were reduced. The assays for the remaining enzymes were not performed.
Histochemical outcome: Analogous results were seen in the histochemical assays. The staining capabilities of alkaline phosphatase, lactat- and malat-dehydrogenase were reduced whereas acid phosphatase showed a moderate staining. The staining capabilities of all other enzymes were strong.
The saline-microwave technique allows a good histochemical as well as a good biochemical investigation of animal tissue.

研究の種別:

研究助成