研究のタイプ: 医学／生物学の研究 (experimental study)

[マイクロ波放射に対するモノアラガイ神経細胞のバースト反応] med./bio.

Bursting responses of Lymnea neurons to microwave radiation

著者: Bolshakov MA, Alekseev SI
掲載誌: Bioelectromagnetics 1992; 13 (2): 119-129
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この研究は、微小電極と電圧クランプ技術を改造して、導波管装置内で900MHz電磁界ばく露中の神経細胞（淡水産巻貝ヨーロッパモノアラガイ）の自発性電気活動とイオン電流を記録する実験装置を開発した。電磁界は、0.5－11パルス／秒で変調したパルス波（PW）、または連続波（CW）を用いた。その結果、PWでは、数W/kgのSARで神経細胞の発火率に高速でバースト様の変化が見られた；この変化はPWにのみ見られたが、変調周波数には依存せず、SAR閾値は0.5 W/kg付近であった；CWでは、同じSARにおいて発火率に影響がなかった；アセチルコリン、ドーパミン、セロトニン、GABAの受容体の活性化は、PWおよびCWで影響が無かった、と報告している。

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研究目的（著者による）

The study is concerned with the appearance of bursting in the spontaneous activity of pacemaker neurons in response to pulsed waves and continuous wave radiation by microwaves.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for up to 15 min
  • SAR: 4 W/kg mean (0.5-4 W/kg)
ばく露2: 900 MHz
Modulation type: CW
ばく露時間: continuous for up to 10 min
  • SAR: 15 W/kg mean (0.5-15 W/kg)

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for up to 15 min
Modulation
Modulation type pulsed
Duty cycle 4 %
Repetition frequency 100 Hz
Additional information

Pulse rates used were 0.5, 2.5, 6, 16, 40, and 100 Hz.
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The experimental techniques and systems were identical to those described in detail by Alekseev et al. [1986].
ばく露装置の詳細 The holding chamber was inserted into the center of a waveguide through an access port that was covered by a metallic mesh (1 x 1 mm) during the experiment.
Additional information The temperature of the preparation was controlled by a flow of the medium through the chamber and did not rise by more than 0.1 °C up to an SAR of 20 W/kg. A desired increase of temperature was achieved by heating the medium by 3 °C and regulating the flow rate.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 4 W/kg mean 測定値 - 0.5-4 W/kg
測定および計算の詳細

To determine SARs, the increase of temperature of the medium at different output powers was measured with a thermistor probe. SARs between 2 and 4 W/kg were measured during exposure, and lower values were extrapolated from the power vs. SAR plot.

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for up to 10 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
パラメータ
測定量 値 種別 Method Mass 備考
SAR 15 W/kg mean 測定値 - 0.5-15 W/kg

Reference articles

  • Alekseev SI et al. (1986): [軟体動物のニューロンのカルシウム電流に対するデシメータ波長の電磁界放射の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

It was found that pulsed waves but not continuous waves elicit bursting responses of neurons. Within the limits of experimental error the effect did not depend on the modulation frequency, but it was sensitive to the SAR. Mediator-induced, current activation of acetylcholine, serotonin, dopamine, or GABA receptors of the neuronal soma was not altered during exposures (continuous wave or pulsed waves) and could not have been responsible for the bursting effect.

研究の種別:

研究助成

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