研究のタイプ: 医学／生物学の研究 (experimental study)

[ラットの脳のアデノシン受容体に対するパルス電磁界ばく露の影響] med./bio.

Effect of pulsed electromagnetic field exposure on adenosine receptors in rat brain

著者: Varani K, Vincenzi F, Targa M, Corciulo C, Fini M, Setti S, Cadossi R, Borea PA
掲載誌: Bioelectromagnetics 2012; 33 (4): 279-287
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この論文は、ラットの大脳皮質および皮質ニューロンのアデノシン受容体（ARs）に対するパルス電磁界（PEMFs）の影響を調べている。PEMFsばく露は、大脳皮質組織の塊、単一の皮質ニューロン、さらにそれから単離された細胞膜切片について行った。その結果、単離された細胞膜と比較して、丸ごとの組織または細胞に対してPEMFは異なった生物学的影響を持つことが示されたと結論している。

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研究目的（著者による）

To study the effects of a pulsed electromagnetic field exposure on adenosine recptors A1 and A2A in the rat cerebral cortex and cortical neurons.

詳細情報

Adenosine, an endogenous modulator of a wide range of biological functions, interacts with at least four cell surface receptor subtypes (as A1, A2A, A2B and A3 adenosine receptors). A1 and A2A adenosine receptors are highly expressed in the central nervous system compared to the other receptor subtypes.
The brain, cerebral cortex and membranes were prepared from male Sprague-Dawley rats.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 75 Hz
Modulation type: pulsed
ばく露時間: continuous for 2 h, 4 h, 6 h or 8 h
  • 磁束密度: 1.5 mT peak (± 0,2 mT (in air at the level of the biological samples))
ばく露2: 75 Hz
Modulation type: pulsed
ばく露時間: continuous for 2 h, 4 h, 6 h or 8 h
  • 磁束密度: 3 mT peak (± 0,2 mT (in air at the level of the biological samples))

ばく露1

主たる特性
周波数 75 Hz
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 continuous for 2 h, 4 h, 6 h or 8 h
Modulation
Modulation type pulsed
Pulse width 1.3 ms
Duty cycle 0.1 %
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 pair of rectangular horizontal coils (14 cm x 23 cm), each made of 1400 turns of copper wire placed opposite to each other; the biological samples (culture in flasks (9,2 cm x 8,2 cm with 10 ml of medium) or brain in a beaker (containing physiological oxygenated buffer)) were placed between the coils, so that the plane of the coils was perpendicular to the culture flasks
Sham exposure A sham exposure was conducted.
Additional information coils were powered by a PEMF generator system
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 1.5 mT peak 測定値 - ± 0,2 mT (in air at the level of the biological samples)
Additional parameter details

the corresponding peak amplitude of the induced electric voltage was 2 ± 0.5 mV

測定および計算の詳細

peak values measured between the two coils in air had a maximum variation of 1% in the whole area in which the culture flasks were placed

ばく露2

主たる特性
周波数 75 Hz
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 continuous for 2 h, 4 h, 6 h or 8 h
Modulation
Modulation type pulsed
Pulse width 1.3 ms
Duty cycle 0.1 %
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 3 mT peak 測定値 - ± 0,2 mT (in air at the level of the biological samples)
Additional parameter details

the corresponding peak amplitude of the induced electric voltage was 2 ± 0.5 mV

測定および計算の詳細

peak values measured between the two coils in air had a maximum variation of 1% in the whole area in which the culture flasks were placed

Reference articles

  • De Mattei M et al. (2009): [ウシ滑膜線維芽細胞内のプロスタグランジンE2放出を抑制するアデノシン類似体および電磁界]
  • Varani K et al. (2008): [低周波数低エネルギーパルス電磁界にばく露されたウシ軟骨細胞および線維芽細胞様滑膜細胞内のアデノイシン受容体の特性評価]
  • Varani K et al. (2003): [ヒト好中球内のA(3)アデノシン受容体の変化と低周波数電磁界]
  • Varani K et al. (2002): [ヒトの好中球のアデノシンA2A受容体に対する低周波電磁界の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

Affinity values were not affected by pulsed electromagnetic field (PEMF) exposure, either at 1.5 or 3 mT. The PEMF exposure of the intact rat cerebral cortex or cortical neurons at 1.5 mT mediated a transient and significant increase in A2A adenosine receptors density after 4 h and 6 h of exposure and returned to values similar to controls after 8 h. In addition, PEMF exposure of the cerebral cortex and cortical neurons at 3 mT upregulated A2A adenosine receptors after 2 h and 4 h. The exposure of cortex membranes at 1.5 and 3 mT induced an increase in A2A adenosine receptor density after 2 h and was constant at all exposure times investigated.
PEMF exposure did not modify binding parameters of A1 adenosine receptors, such as affinity and density at 1.5 or 3 mT.
In cortical neurons, mRNA levels of A1 and A2A adenosine receptors were not affected by PEMF exposure for the times and intensities investigated. These results suggest that the PEMF-induced alteration of A2A adenosine receptors comprises the expression of receptors at the surface of the cell membrane, but not the transcriptional level.
In conclusion, the data clearly suggest a time and intensity-dependent effect of PEMFs on A2A adenosine receptors density in rat brain and cortical neurons. The PEMF-mediated upregulation of A2A adenosine receptors was constant in brain membranes and transient in the whole brain or intact cortical neurons.

研究の種別:

研究助成

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