研究のタイプ: 医学/生物学の研究 (experimental study)

[大腸菌細胞と低強度ミリ波との間の共振的な相互作用の標的としての染色体DNA] med./bio.

Chromosome DNA as a target of resonant interaction between Escherichia coli cells and low-intensity millimeter waves.

掲載誌: Electro Magnetobiol 1992; 11 (2): 97-108

この研究は、異常粘度時間依存性(AVTD)法を用いて、非熱レベルのマイクロ波大腸菌(Escherichia coli)細胞のゲノム構造状態(GCS)に及ぼす影響を調べた。20GyのX線照射を受けた細胞誘導されたGCS変化の修復が、直線偏波マイクロ波(51.62-51.84GHz帯の7つの周波数)によって阻害されることが示された、と報告している。

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研究目的(著者による)

To study the influence of millimeter waves on the process of the genome conformational state repair in E. coli (with and without preceding exposure to X-rays).
To determine the sign of effective polarization (linear, left-handed circular, and right-handed circular) and parameters of resonances in the 51.62-51.84 GHz band, the effect of differently polarized millimeter waves on the genome conformational state of intact cells and cells exposed to X-rays at a dose of 20 Gy was studied (see "Background/further details"). Other radiation doses of 0.01-3 Gy were also conducted to determine the minimal effective dose.

詳細情報

In a previous study (see publication 10177), linearly polarized microwaves in two frequency bands (41.25-41.50 GHz and 51.62-51.84 GHz) suppressed repair of genome conformational state in cells exposed to 20-30 Gy of X-rays and in both bands, the effect was frequency dependent with resonance frequencies of f = 41.32 GHz and f = 51.76 GHz. In subsequent studies (publication 15590) it was established that right- and left-handed polarized electromagnetic irradiation have different efficiency at resonance frequencies.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 51.62–51.84 GHz
ばく露時間: continuous for 10 min after X-ray irradiation
  • SAR: 6 µW/g spatial average (cf. remarks) (at 1 µW/cm²)
  • 電力密度: 100 µW/cm² spatial average
ばく露2: 51.76 GHz
ばく露時間: continuous for 5 min after X-ray irradiation
  • SAR: 6 µW/g spatial average (cf. remarks) (at 1 µW/cm²)
  • 電力密度: 100 µW/cm² spatial average
ばく露3: 51.76 GHz
ばく露時間: continuous for 5 min
  • SAR: 6 µW/g spatial average (cf. remarks) (at 1 µW/cm²)
  • 電力密度: 100 µW/cm² spatial average
ばく露4: 51.68–51.85 GHz
ばく露時間: continuous for 5 min
  • SAR: 6 µW/g spatial average (cf. remarks) (at 1 µW/cm²)
  • 電力密度: 100 µW/cm² spatial average

General information

for further information on the exposure setup see: Belyaev, I. Ya., Shcheglov, V. S., and Alipov, Ye. D. "Selection rules on helicity during discrete transitions of the genome conformational state in intact and X-rayed cells of E. coli in millimeter rage of electromagnetic field." In: Charge and Field Effects in Biosystems-3 (M. J. Allen, S. F. Cleary, A. E. Sowers, and D. D. Shillady, eds.), Birkhäuser, Boston, 1992, pp. 115-126 cells were treated in four groups: i) control ii) X-ray irradiation iii) EMF iv) X-rays + EMF

ばく露1

主たる特性
周波数 51.62–51.84 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 10 min after X-ray irradiation
ばく露装置
ばく露の発生源/構造
  • 詳細不明
Additional information linear, left-handed circular and right-handed circular polarized electromagnetic field used
パラメータ
測定量 種別 Method Mass 備考
SAR 6 µW/g spatial average 測定値 cf. remarks at 1 µW/cm²
電力密度 100 µW/cm² spatial average 測定値 - -

ばく露2

主たる特性
周波数 51.76 GHz
タイプ
  • electromagnetic field
偏波
  • circular
ばく露時間 continuous for 5 min after X-ray irradiation
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Additional information left-handed circular and right-handed circular polarized electromagnetic field used
パラメータ
測定量 種別 Method Mass 備考
SAR 6 µW/g spatial average 測定値 cf. remarks at 1 µW/cm²
電力密度 100 µW/cm² spatial average 測定値 - -

ばく露3

主たる特性
周波数 51.76 GHz
タイプ
  • electromagnetic field
偏波
  • circular
ばく露時間 continuous for 5 min
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Additional information left-handed circular polarized electromagnetic field used
パラメータ
測定量 種別 Method Mass 備考
SAR 6 µW/g spatial average 測定値 cf. remarks at 1 µW/cm²
電力密度 100 µW/cm² spatial average 測定値 - -

ばく露4

主たる特性
周波数 51.68–51.85 GHz
タイプ
  • electromagnetic field
偏波
  • circular
ばく露時間 continuous for 5 min
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Additional information left-handed and right-handed circular polarized electromagnetic field used
パラメータ
測定量 種別 Method Mass 備考
SAR 6 µW/g spatial average 測定値 cf. remarks at 1 µW/cm²
電力密度 100 µW/cm² spatial average 測定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

At all the frequencies that were studied, right-handed-polarized microwaves effectively influenced the genome conformational state (GCS) of X-radiated cells, whereas left-handed polarization was ineffective.
Conversely, right-handed polarization was ineffective and left-handed polarized electromagnetic irradiation influenced the GCS when intact cells were exposed to microwaves.
It was shown that the effects of left- and right-handed electromagnetic irradiation on the cells become the same at 50 cGy. This dose is too small to damage any cellular structures except DNA. The results suggest that the target of resonant interaction between E. coli cells and millimeter waves appears to be chromosomal DNA.

研究の種別:

研究助成

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