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To investigate the possible changes induced by the exposure to a radiofrequency electromagnetic field in protein expression profile in human chorionic villous tissue (part of the placenta) of early pregnancy.
The women, gestational age about 50-60 days, were randomly divided into an exposure and a control group. After 20 minutes of exposure, an abortion was performed and the chorionic villous tissue was taken through the cervix. Tissue, collected from 6 exposed women and from 6 control women, was taken for proteome analysis.
The women underwent terminations of the pregnancies for psychological reasons.
ばく露時間: continuous for 20 min. (according to "materials and methods" section) or 1 h (according to abstract)?
|ばく露時間||continuous for 20 min. (according to "materials and methods" section) or 1 h (according to abstract)?|
|ばく露装置の詳細||lower abdomens of the pregnant women were exposed by a commercially available cell phone (navel region); cell phone was in talk mode; distance of cell phone to abdomen 2.5 cm (according to "materials and methods" section) or 10 cm (according to abstract)?|
|Sham exposure||A sham exposure was conducted.|
Approximately, 2000 spots were detected in the two-dimensional gel electrophoresis. Among them, 9 proteins of the exposed group were significantly down-regulated and 6 proteins were significantly up-regulated compared to the control group. Out of these 15 spots, 12 proteins were identified. The protein expression of 3 proteins was verified using Western blot.
The authors conclude that electromagnetic fields from cell phones could alter the protein expression profile of chorionic villous tissue of the early pregnancy.