研究のタイプ: 医学/生物学の研究 (experimental study)

[低周波振幅変調マイクロ波界はシナプトソームからのカルシウム流出率を変えた] med./bio.

Low frequency amplitude modulated microwave fields change calcium efflux rates from synaptosomes.

掲載誌: Bioelectromagnetics 1982; 3 (3): 309-322

低周波マイクロ波電界(450Hz, 0.5mW/cm^2, 変調16Hz, 60Hz)によるラットシナプトゾームからのCa流出率変化を調べた.16Hz正弦波電界ばく露した場合,割合が38%増加した.非正弦波,あるいは60Hz正弦波電界では影響がなかった.

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研究目的(acc. to editor)

To investigate the effects of a 450 MHz (16 Hz AM, 60 Hz AM, or CW) microwave exposure in vitro on the calcium efflux in rat synaptosomes.

詳細情報

Electron microscopy was used to determinate the integrity of the synaptosome preparation. To examine the calcium efflux during exposure a continuous perfusion technique was used.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 450 MHz
Modulation type: CW
ばく露時間: continuous for 10 min
ばく露2: 450 MHz
Modulation type: AM
ばく露時間: continuous for 10 min
ばく露3: 450 MHz
Modulation type: AM
ばく露時間: continuous for 10 min

ばく露1

主たる特性
周波数 450 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 10 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
チャンバの詳細 A hemispheric plastic Millipore filter holder having a base diameter of 25 mm and a volume of 1 cm³ was mounted on a Plexiglas platform in the center of a Crawford cell with the filter 5 cm below the top of the chamber.
ばく露装置の詳細 The filter was half-filled with temperature controlled solution at 31 °C delivered from a syringe at 1 ml/min throughout the perfusion. Perfusate-collecting vials were placed in a cardboard box with 6-mm walls on the septum and were moved manually one position every minute.
Additional information Duplicate samples were prepared from the same incubation; one served as control and the other one was exposed to MW fields during perfusion. Both syringes were mounted on the same pump, and temperature was maintained by the same circulator. The effect of temperature alone was studied by perfusion at 25, 31, 33, and 35 °C. For stimulation by calcium, a concentrated CaCl2 solution was injected at a rate of 10µl/min for 5 min. Choline chloride injection served as control.
パラメータ
測定量 種別 Method Mass 備考
電力密度 0.5 mW/cm² peak 測定値 - -
電界強度 43 V/m peak 測定値 - in air

ばく露2

主たる特性
周波数 450 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 10 min
Modulation
Modulation type AM
Modulation frequency 16 Hz
Modulation depth 75 %
Additional information

sinusoidal

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電力密度 0.5 mW/cm² peak 測定値 - -

ばく露3

主たる特性
周波数 450 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 10 min
Modulation
Modulation type AM
Modulation frequency 60 Hz
Modulation depth 75 %
Additional information

sinusoidal

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電力密度 0.5 mW/cm² peak 測定値 - -

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The calcium efflux was increased by the 450 MHz 16 Hz AM modulated microwave exposure, whereas exposure to CW or 60 Hz AM modulated fields did not lead to similar changes.

研究の種別:

研究助成

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