低周波マイクロ波電界（450Hz, 0.5mW/cm^2, 変調16Hz, 60Hz）によるラットシナプトゾームからのCa流出率変化を調べた．16Hz正弦波電界をばく露した場合，割合が38％増加した．非正弦波，あるいは60Hz正弦波電界では影響がなかった．
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To investigate the effects of a 450 MHz (16 Hz AM, 60 Hz AM, or CW) microwave exposure in vitro on the calcium efflux in rat synaptosomes.
Electron microscopy was used to determinate the integrity of the synaptosome preparation. To examine the calcium efflux during exposure a continuous perfusion technique was used.
| 周波数 | 450 MHz |
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| タイプ |
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| 特性 |
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| ばく露時間 | continuous for 10 min |
| Modulation type | CW |
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| ばく露の発生源／構造 | |
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| チャンバの詳細 | A hemispheric plastic Millipore filter holder having a base diameter of 25 mm and a volume of 1 cm³ was mounted on a Plexiglas platform in the center of a Crawford cell with the filter 5 cm below the top of the chamber. |
| ばく露装置の詳細 | The filter was half-filled with temperature controlled solution at 31 °C delivered from a syringe at 1 ml/min throughout the perfusion. Perfusate-collecting vials were placed in a cardboard box with 6-mm walls on the septum and were moved manually one position every minute. |
| Additional information | Duplicate samples were prepared from the same incubation; one served as control and the other one was exposed to MW fields during perfusion. Both syringes were mounted on the same pump, and temperature was maintained by the same circulator. The effect of temperature alone was studied by perfusion at 25, 31, 33, and 35 °C. For stimulation by calcium, a concentrated CaCl2 solution was injected at a rate of 10µl/min for 5 min. Choline chloride injection served as control. |
The calcium efflux was increased by the 450 MHz 16 Hz AM modulated microwave exposure, whereas exposure to CW or 60 Hz AM modulated fields did not lead to similar changes.
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