[活性酸素種感受性のナノ粒子による間質細胞由来因子-1αの標的化放出は骨髄間質細胞の化学走性及び帰巣性、ならびに電撃傷による血管損傷の修復を生じる] med./bio.

Targeted release of stromal cell-derived factor-1α by reactive oxygen species-sensitive nanoparticles results in bone marrow stromal cell chemotaxis and homing, and repair of vascular injury caused by electrical burns

影響評価項目

• Effect and mechanisms of healing of vascular injury caused by electrical burns with SDF-1α-PPADT nanoparticles

ばく露

• • 50/60 Hz (AC)
  • （接触）電流
• • 電撃傷／感電死
• • low voltage

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方法 影響評価項目／測定パラメータ／方法

• 形態学/組織学的変化: histopathological changes of wound tissue (hematoxylin-eosin stain and immunohistochemical stain of CD31 (marker for angiogenesis); microscopy), reactive oxygen species in wound tissue (fluorescence microscopy)
• 分子生合成: gene expression of antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase in wound tissue (RT-PCR), protein expression of SDF-1α in wound tissue (ELISA)
• verification of ROS-sensitive targeted release of SDF-1α from nanoparticels in mice in vivo (injection of fluorescence-labeled SDF-1α or SDF-1α-PPADT; fluorescence imaging system) and in wound tissue in vitro (injection of SDF-1α or SDF-1α-PPADT; ELISA); directional chemotaxis and homing of BMSCs to wound tissue (injection of fluorescence-labeled BMSCs and SDF-1α or SDF-1α-PPADT and immunofluorescence stain of CD31; fluorescence microscopy); histopathological effects of SDF-1α-PPADT nanoparticles on vascular repair (injection of rats with SDF-1α or SDF-1α-PPADT, hematoxylin-eosin stain and immunohistochemical stain of CD31 of wound tissue; microscopy)

研究助成

• National Natural Science Foundation (NSFC), China
• Shanghai Municipal Education Commission, China

関連論文

• Saputro I et al. (2018): [電撃熱傷における広範な組織破壊の防止に対するN-アセチルシステインの影響]
• Prindeze NJ et al. (2014): [電気接触傷害後に検出される局所的神経血管炎症およびアポトーシス]
• Shupp JW et al. (2012): [電撃傷を実験的に与えるシステムを用いた電撃傷への局所的および全身的イン・ビボ応答の検査]
• Laberge LC et al. (1984): [実験中の電撃傷：低電圧。]