Targeted release of stromal cell-derived factor-1α by reactive oxygen species-sensitive nanoparticles results in bone marrow stromal cell chemotaxis and homing, and repair of vascular injury caused by electrical burns.
He F, Luo PF, Tang T, Zhang F, Fang H, Ji SZ, Sun Y, Wu GS, Pan BH, Huo ZB, Wang GY, Xia ZF
形態学/組織学的変化: histopathological changes of wound tissue (hematoxylin-eosin stain and immunohistochemical stain of CD31 (marker for angiogenesis); microscopy), reactive oxygen species in wound tissue (fluorescence microscopy)
分子生合成: gene expression of antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase in wound tissue (RT-PCR), protein expression of SDF-1α in wound tissue (ELISA)
verification of ROS-sensitive targeted release of SDF-1α from nanoparticels in mice in vivo (injection of fluorescence-labeled SDF-1α or SDF-1α-PPADT; fluorescence imaging system) and in wound tissue in vitro (injection of SDF-1α or SDF-1α-PPADT; ELISA); directional chemotaxis and homing of BMSCs to wound tissue (injection of fluorescence-labeled BMSCs and SDF-1α or SDF-1α-PPADT and immunofluorescence stain of CD31; fluorescence microscopy); histopathological effects of SDF-1α-PPADT nanoparticles on vascular repair (injection of rats with SDF-1α or SDF-1α-PPADT, hematoxylin-eosin stain and immunohistochemical stain of CD31 of wound tissue; microscopy)