研究のタイプ: 医学/生物学の研究 (experimental study)

[ラット脳のシナプトソームのホスホイノシタイドへの32Piの取り込みのマイクロ波による刺激] med./bio.

Microwave induced stimulation of 32Pi incorporation into phosphoinositides of rat brain synaptosomes.

掲載誌: Radiat Environ Biophys 1989; 28 (3): 223-234

この研究は、10mW / cm 2以上の電力密度マイクロ波放射ばく露が、シナプトソームでのホスホイノシチドへの32Pi取り込み刺激することについて調べた。その結果、ホスファチジルイノシトール(PI)およびホスファチジン酸(PA)と比較して、32 Pi取り込みの程度は、ホスファチジルイノシトール-4-リン酸(PIP)およびホスファチジルイノシトール-4,5-ビスホスフェート(PIP2)においてより大きく増強される;他の脂質も32Piを取り込むが、マイクロ波放射ばく露によりそれらが有意に変することはなかった、と報告している。

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研究目的(著者による)

To study the effect of microwave exposure on the metabolism of synaptosomal membrane phospholipids. 32Pi-incorporation into phosphoinositides was studied.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.8 GHz
Modulation type: pulsed
ばく露時間: 30 min

ばく露1

主たる特性
周波数 2.8 GHz
タイプ
  • electromagnetic field
ばく露時間 30 min
Modulation
Modulation type pulsed
Pulse width 1 µs
Packets per second 1
Repetition frequency 350 Hz
ばく露装置
ばく露の発生源/構造
Distance between exposed object and exposure source 2 m
ばく露装置の詳細 samples exposed in 16 x 100 mM glass tubes
Additional information distance between horn and samples : 2 m for PD 100 and 300 W/m² and 6 m for 10 W/m²
パラメータ
測定量 種別 Method Mass 備考
電力密度 10 W/m² - 備考を参照のこと。 - 100, 300 W/m²

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Irradiation of synaptosomes at a power density of 10 mW/cm² or more produced stimulation of the 32Pi-incorporation into phosphoinositides. The extent of 32Pi-incorporation was found to be much more pronounced in phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2) as compared to phosphatidylinositol (PI) and phosphatidic acid (PA). Inclusion of 10 mM lithium (Li+) in the medium decreased the basal labeling of PIP2, PIP and PI and increased PA labeling. Li+ also inhibited the microwave stimulated PIP2, PIP and PI labeling but had no effect on PA labeling. Calcium ionophore (A23187) inhibited the basal and microwave stimulated 32Pi labeling of PIP and PIP2, stimulated basal labeling of PA and PI and had no effect on microwave stimulated PA and PI labeling. Calcium chelator (EGTA) on the other hand, had no effect on basal labeling of PA and PI, stimulated basal PIP and PIP2 labeling but did not change microwave stimulated labeling of these lipids. Microwave exposure did not produce any significant effect on the endogenous concentrations of the total as well as individual phospholipids of synaptosomes indicating that only phosphoinositide turnover was stimulated.

研究の種別:

研究助成