この研究は、睡眠—覚醒のサイクルにおけるセレトニン（5-HT）ニューロン活性の研究方法へのマイクロ波応用について検討した。1973年にSinhaらが、徐波睡眠中に屠殺して直ちに得た皮質中での5-HT含有量低下を報告して以来、この分野の研究が進められているが、5-HTターンオーバーの死後変化など、研究手法の短所が問題となっている。これらの短所を克服する方法として、数秒で組織酵素を不活化できるマイクロ波固定法の有望性を報告している。
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To study changes in serotonin (5-HT) and its metabolite 5-hydroxyindole acetic acid (5-HIAA) in rat brain during slow-wave sleep and paradoxical sleep.
The microwave fixation method was applied. Rats were sacrified by microwave irradiation of the head (2450 MHz, 5 kW, 2.0 sec) after 10 min of slow-wave sleep or wakefulness or paradoxical sleep.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
2.45 GHz
ばく露時間:
2 s
|
|
| 周波数 | 2.45 GHz |
|---|---|
| タイプ |
|
| ばく露時間 | 2 s |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 電力 | 5 kW | - | - | - | - |
Since the polygraphic records did not change just before exposure and the brains were fixed within 2 sec., the authors concluded that their procedure seems to be better for fixation of the brain at various states of sleep and wakefulness than previous methods (such as KCl injection or brain biopsy). Serotonin content was higher in the cerebral cortex and midbrain during both phases of sleep than during wakefulness.The 5-HIAA content in the hippocampus was higher during slow-wave sleep than during wakefulness. Similar tendencies were revealed in other regions, but not statistically significant. The 5-HIAA content to the cerebral cortex, hippocampus, midbrain and pons and medulla oblongata were lower during paradoxical sleep than during slow-wave sleep.
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