研究のタイプ: 医学/生物学の研究 (experimental study)

[細胞増殖と相関したコヒーレントな興奮の実験的証拠] med./bio.

Experimental evidence for coherent excitations correlated with cell growth.

掲載誌: Nanobiology 1992; 1: 163-176
Journal partially peer-reviewed/unclear

この研究は、出芽酵母(Saccharomyces cerevisiae)の細胞増殖に対するマイクロ波照射の影響を調べた。標準化ミリ波導波管に結合したばく露チャンバには、サーモスタットおよび細胞観察のための走査型顕微鏡のステージに取り付けられた。周波数は41.688〜41.707 GHz、8 kHz変調方形波を用い、ばく露時間は10時間連続で行なった。ばく露を受ける細胞は単層培養された。結果として、マイクロ波ばく露は、酵母の細胞増殖速度を、周波数と強度に依存する形で変化させた;細胞増殖は、10〜20%の範囲で増加または減少した、と報告している。

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研究目的(著者による)

To investigate the influence of microwave irradiation on the cell growth of the yeast Saccharomyces cerevisiae.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 41.6965 GHz
ばく露時間: continuous for 10 h
ばく露2: 41.6965 GHz
ばく露時間: continuous for 10 h

General information

A total of 37 growth experiments were conducted at 1 nW/cm², 27 with and 10 without MW radiation. Further 33 runs were performed at 5 pW/cm².

ばく露1

主たる特性
周波数 41.6965 GHz
タイプ
  • electromagnetic field
偏波
  • linear
ばく露時間 continuous for 10 h
Additional information Several frequencies between 41.688 and 41.707 GHz were applied.
Modulation
Modulation type cf. additional information
Additional information

modulated at 8 kHz, square wave

ばく露装置
ばく露の発生源/構造
  • radiating slot of a gold-coated quartz needle
チャンバの詳細 The thermostatted metallic irradiation chamber, coupled by a flexible waveguide with a standardised millimeter wave set-up [Grundler et al., 1983], was mounted on the stage of a scanning microscope.
ばく露装置の詳細 The radiation was guided through a tapered gold-coated quartz needle broadened by quartz carrier plates on both sides and radiated out of a slot (0.4 mm x 1.7 mm) on which the cells were spread in a monolayer. Yeast cells were fixed to the lower surface of a thin transparent agar layer (0.2 mm) filling the inner part of a smaller plastic ring. The agar layer and the cells rested on the quartz needle and the carrier plates. At the opposite surface of the quartz needle, a broader opening for microscopic viewing was covered with a fine metallic mesh suppressing RF leakage.
Additional information The MW field in the radiating slot was monomodal, and the EF was polarized in the y direction, surrounded by the MF in the x-z plane.
パラメータ
測定量 種別 Method Mass 備考
電力密度 1 nW/cm² mean 測定値 - -
SAR 0.04 µW/g mean - - -

ばく露2

主たる特性
周波数 41.6965 GHz
タイプ
  • electromagnetic field
偏波
  • linear
ばく露時間 continuous for 10 h
Additional information Several frequencies between 41.688 and 41.704 GHz were applied.
Modulation
Modulation type cf. additional information
Additional information

modulated at 8 kHz, square wave

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電力密度 0.005 nW/cm² mean 測定値 - -
SAR 0.2 nW/g mean - - -

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中

研究の主なアウトカム(著者による)

Microwave exposure affects the cell growth rate of the yeast S. cerevisiae in a frequency and intensity dependent manner. Cell growth was either increased or decreased in a range of 10-20%.

研究の種別:

研究助成

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