方法 ：洗浄・濃縮されたヒトの射出精液を900 MHz GSM携帯電話パルス波にばく露させ、対照群と比較した。SARレベルは2.0または5.7 W/kg とした。精子細胞のミトコンドリア膜電位の変化はフローサイトメトリを用いて解析した。精子運動性はコンピュータ精子分析装置で測定した。結果 ：ミトコンドリア膜電位への影響は観察されなかった。運動性については、SAR値2.0 W/kgでは全ての運動性変数に変化はなかったが、5.7 W/kgでは、ばく露後の時間経過（ばく露直後、2時間後、24時間後）によって、直線運動速度と精子頭部振動頻度に有意な影響が見られた。RFばく露チャンバー内を冷却し、温度制御されていたため、熱の影響は排除されたと思われる。
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This study was performed to examine the effect of pulsed 900 MHz radiofrequency electromagnetic field exposure on human sperm motility in vitro as indicator of male fertility.
Semen samples were collected from healthy, nonsmoking donors (n=12) by masturbation after 2-3 days of sexual abstinence. The semen parameters of these samples were within the normal range of the WHO criteria. Samples were exposed to two different specific absorption rates (2.0 and 5.7 W/kg) and run in duplicate.
The sperm motility was assessed by objective motility measurement and by the inner mitochondrial membrane potential as its decrease is highly correlated with diminished sperm motility and fertilisation potential.
|ばく露時間||continuous for 1 h|
|Pulse width||0.577 ms|
|Repetition frequency||217 Hz|
|チャンバの詳細||The exposure chamber was positioned vertically inside a CO2 incubator.|
|ばく露装置の詳細||Two 55-mm glass Petri dishes were placed inside the chamber on a 9-mm thin rectangular glassfibre-moulded, temperature controlled waterbed. The surface of the culture medium was aligned parallel to the E-field vector.|
|Additional information||Cell temperatures measured during exposure ranged from 36.7 to 37.3 °C at 5.7 W/kg and from 36.8 to 37.2 °C at 2 W/kg.|
There was no effect of pulsed 900 MHz GSM irradiation on the mitochondrial membrane potential. Additionally, no changes for all kinematic sperm motility parameters assessed at a specific absorption rate of 2.0 W/kg were observed. However, two kinematic parameters of sperm's velocity and motion were significantly decreased after the exposure at the specific absorption rate of 5.7 W/kg.
Further research is relevant to clarify whether the observed effects at the specific absorption rate of 5.7 W/kg are artefacts or cellular mechanisms verifiable.