Medical/biological study (experimental study)

Disturbance of cell proliferation in response to mobile phone frequency radiation med./bio.

By: Trosic I, Pavicic I

Published in: Arh Hig Rada Toksikol 2009; 60 (1): 109-115

Aim of study (acc. to author)

To determine the influence of mobile phone exposure on the proliferation, cytoskeleton structure, and mitotic index of V79 cells.

Background/further details

Positive control cells were treated with colchicine (concentration of 0.1 mmol/L).

Endpoint

cell viability/cell division/proliferation: cell proliferation, mitotic index
morphol./histopathol. changes: microtubule protein structure

Exposure

- CW continuous wave
- mobile communications
- mobile phone
- digital mobile phone

Exposure	Parameters
Exposure 1: 935 MHz Exposure duration: continuous for 1 h, 2 h and 3 h	electric field strength: 8.2 V/m SAR: 0.12 W/kg spatial average

Exposure 1

Main characteristics

Frequency	935 MHz
Type	electromagnetic field
Exposure duration	continuous for 1 h, 2 h and 3 h

Exposure setup

Exposure source	GTEM cell
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
electric field strength	8.2 V/m	-	-	-	-
SAR	0.12 W/kg	spatial average	calculated	-	-

Exposed system:

intact cell/cell culture
V79 (Chinese hamster fibroblast cells)

Methods Endpoint/measurement parameters/methodology

cell viability/cell division/proliferation: cell proliferation (cell counting with hemocytometer after 24h, 48h, 72h, and 96h; light microscopy), mitotic index (after 24h; light microscopy)
morphol./histopathol. changes: cell morphology and microtubule protein structure (immunocytochemistry; fluorescence microscopy)

Investigated system:

intact cell/cell culture
cell smears

Time of investigation:

after exposure

Main outcome of study (acc. to author)

In comparison to the control cells, cell proliferation declined significantly in cells exposed for three hours 72h after irradiation. Microtubule structure (fibre damage) was significantly altered immediately after exposure. The mitotic index in exposed cells did not differ from negative controls.
The results indicate that cell proliferation kinetics may depend on microtubule impairment after exposure to a mobile phone.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Ministry of Science, Education and Sports, Republic of Croatia

Lee SS et al. (2014): Influence of smartphone Wi-Fi signals on adipose-derived stem cells
Ballardin M et al. (2011): Non-thermal effects of 2.45GHz microwaves on spindle assembly, mitotic cells and viability of Chinese hamster V-79 cells
Atasoy A et al. (2009): The effects of electromagnetic fields on peripheral blood mononuclear cells in vitro
Pavicic I et al. (2008): Impact of 864 MHz or 935 MHz radiofrequency microwave radiation on the basic growth parameters of V79 cell line
Pavicic I et al. (2008): In vitro testing of cellular response to ultra high frequency electromagnetic field radiation
Tsai MT et al. (2007): Pulsed electromagnetic fields affect osteoblast proliferation and differentiation in bone tissue engineering
Pavicic I et al. (2006): Comparison of 864 MHz and 935 MHz microwave radiation effects on cell culture
Pavicic I et al. (2006): Influence of 864 MHz electromagnetic field on growth kinetics of established cell line
Capri M et al. (2004): In vitro exposure of human lymphocytes to 900 MHz CW and GSM modulated radiofrequency: studies of proliferation, apoptosis and mitochondrial membrane potential
Pacini S et al. (2002): Exposure to global system for mobile communication (GSM) cellular phone radiofrequency alters gene expression, proliferation, and morphology of human skin fibroblasts
Donnellan M et al. (1997): Effects of exposure to electromagnetic radiation at 835 MHz on growth, morphology and secretory characteristics of a mast cell analogue, RBL-2H3
French PW et al. (1997): Electromagnetic radiation at 835 MHz changes the morphology and inhibits proliferation of a human astrocytoma cell line