Study type: Medical/biological study (experimental study)

Reactive oxygen species levels and DNA fragmentation on astrocytes in primary culture after acute exposure to low intensity microwave electromagnetic field. med./bio.

Published in: Neurosci Lett 2010; 473 (1): 52-55

Aim of study (acc. to author)

To study the effects of acute exposure to electromagnetic fields in the microwave range on primary rat neocortical astroglial cell cultures.

Background/further details

Primary cultures of astrocytes were prepared from the neocortex of newborn rat brains (Wistar rats).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Modulation type: CW
Exposure duration: continuous for 5 min., 10 min. or 20 min.
Exposure 2: 900 MHz
Modulation type: AM
Exposure duration: continuous for 5 min., 10 min. or 20 min.

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Exposure duration continuous for 5 min., 10 min. or 20 min.
Modulation
Modulation type CW
Exposure setup
Exposure source
Distance between exposed object and exposure source 60 cm
Setup double horn antenna placed above the samples
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 10 V/m effective value measured - -
power density 0.26 W/m² - - - -

Exposure 2

Main characteristics
Frequency 900 MHz
Type
Exposure duration continuous for 5 min., 10 min. or 20 min.
Modulation
Modulation type AM
Modulation frequency 50 Hz
Modulation depth 100 %
Exposure setup
Exposure source
Distance between exposed object and exposure source 60 cm
Setup double horn antenna placed above the samples
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
electric field strength 10 V/m effective value measured - -
power density 0.26 W/m² - - - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

Significant increases in reactive oxygen species levels and DNA fragmentation were found only in the astrocytes exposed to the modulated electromagnetic field for 20 minutes. No effects were detected when shorter exposure durations or continuous wave exposures were used. The exposure conditions allowed the exclusion of any possible thermal effect.
The data didn't show any significant changes in the release of lactate dehydrogenase, neither for exposure to modulated nor to not modulated (CW) electromagnetic fields.
The data demonstrated, that acute exposure to low intensity microwave electromagnetic fields induces reactive oxygen species production and DNA fragmentation in astrocytes in primary cultures.

Study character:

Study funded by

Related articles