To examine the effects of extremely low frequency electric fields on oxidative stress, nitrosative stress parameters and antioxidant enzyme activities in the liver tissue of guinea pigs. The study also adresses whether individual and combined application of N-acetyl-L-cysteine and epigallocatechin-3-gallate decreases the harmful effects of extremely low frequency electric fields.
80 male guina pigs were randomly divided into eight groups (10 each): 1) sham exposure group, 2) N-acetyl-L-cysteine group (NAC), 3) epigallocatechin-3-gallate group (EGCG), 4) NAC + EGCG group, 5) electric field exposure group, 6) NAC + electric field exposure group, 7) EGCG + electric field group, and 8) NAC + EGCG + electric field group. All exposures started at 09:00 h and ended at 17:00 h for seven consecutive days.
Exposure duration: 8 h/d for 7 days
animals were treated in the following groups: i) sham exposure ii) sham exposure + 30 mg/kg N-acetyl-L-cysteine (NAG) iii) sham + 25 mg/kg epigallocatechin-gallate (EGCG) iv) sham + NAG + EGCG v) exposure to electric field vi) exposure to electric field + NAC vii) exposure to electric field + EGCG viii) exposure to electric field + NAC + EGCG
|Exposure duration||8 h/d for 7 days|
|electric field strength||12 kV/m||-||measured and calculated||-||-|
The results indicated a significant increase in the levels of oxidant products (malondialdehyde, nitrate, nitrite, total level of nitric oxide), and a significant decrease in antioxidant enzyme activities (superoxide dismutase, glutathione peroxidase, myeloperoxidase). The individual or combined application of N-acetyl-L-cysteine and epigallocatechin-3-gallate prior to electric field application resulted in the reduction of oxidative stress.
Taken together, these findings support the suggestion that N-acetyl-L-cysteine and epigallocatechin-3-gallate might have hepato-protective effects in extremely low frequency electric field induced oxidative and nitrosative stress.