研究のタイプ: 医学/生物学の研究 (experimental study)

[インビトロ培養のAT478げっ歯類扁平上皮がん細胞株における坑酸化ビタミンEの特性に対する超低周波磁界の影響] med./bio.

Influence of an Extremely Low Frequency Magnetic Field (ELF-EMF) on Antioxidative Vitamin E Properties in AT478 Murine Squamous Cell Carcinoma Culture In Vitro.

掲載誌: Int J Toxicol 2010; 29 (2): 221-230

この研究は、インビトロ培養のAT478げっ歯類扁平上皮がん細胞株に対する、ビタミンE単独、超低周波電磁界(ELF-EMF)単独、およびその組合せばく露の影響を調べた。対照群ビタミンE投与群、(400Hz中心、B=0.11 mT、16分間)、ELF-EMF+ビタミンE投与群を比較した結果、ビタミンE投与群では、抗酸化酵素活性量反応関係をもって増加した;ELF-EMF単独、ELF-EMF+ビタミンE投与群では、スーパーオキサイドデムスターゼアイソザイムとグルタチオンペルオキシダーゼの活性が対照群に比べ上昇した、などの所見を報告している。

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研究目的(著者による)

To study the effects of extremely low frequency electromagnetic fields and antioxidant supplementation (vitamin E), alone or in combination, on antioxidant enzyme activities and level of lipid peroxidation in a carcinoma cell line in vitro.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 3 Hz–3 kHz
ばく露時間: continuous for 16 min

ばく露1

主たる特性
周波数 3 Hz–3 kHz
タイプ
  • magnetic field
ばく露時間 continuous for 16 min
ばく露装置
ばく露の発生源/構造
  • MRS 2000 device
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.11 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Following vitamin E treatment, antioxidant enzyme activity was increased compared with the untreated control group. Extremely low frequency electromagnetic field exposure alone or in combination with vitamin E increased both superoxide dismutase enzyme activities and glutathione peroxidase enzyme activity in comparison to the control group.
The data suggest that extremely low frequency electromagnetic fields alter antioxidative enzyme activities in AT478 tumor cells. This study confirms the role of vitamin E in decreasing susceptibility to lipid peroxidation (decreased levels of malondialdehyde) in AT478 tumor cells.
Further studies should be conducted to determine the influence of extremely low frequency electromagnetic field exposure and vitamin E on cell proliferation processes and chemosensitivity of the cancer cells to commonly used cytostatics causing reactive oxygen species-mediated damage (i.e. high levels of glutathione peroxidase in cancer cells lead to cancer cell survival via effective removal of hydrogen peroxide).

研究の種別:

研究助成

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