Study type: Medical/biological study (experimental study)

Micronucleus induction in Syrian hamster embryo cells following exposure to 50 Hz magnetic fields, benzo(a)pyrene, and TPA in vitro. med./bio.

Published in: Mutat Res Genet Toxicol Environ Mutagen 2001; 495 (1-2): 43-50

Aim of study (acc. to author)

To study possible carcinogenic effects of extremely low frequency electromagnetic fields in mammalian cells in vitro.

Background/further details

Cells were additionally exposed to benzpyrene (as initiator) and/or tetradecanoylphorbol acetate (as cocarcinogen) plus/without extremely low frequency electromagnetic field in order to estimate carcinogenic/cocarcinogenic effects of extremely low frequency electromagnetic field.
Experiments were repeated three to five times.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 50 Hz
Exposure duration: 24, 48, 72 h

General information

cell cultures were treated with i) only EMF ii) EMF + benzpyrene iii) EMF + tetradecanoylphorbol-acetate iv) EMF + benzpyren + tetradecanoylphorbol-acetate

Exposure 1

Main characteristics
Frequency 50 Hz
Type
Waveform
Polarization
Exposure duration 24, 48, 72 h
Exposure setup
Exposure source
Setup pair of parallel Helmholtz coils with a diameter of 400 mm each, 200 mm apart
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 1 mT - measured - -

Reference articles

  • Simko M et al. (1998): Effects of 50 Hz EMF exposure on micronucleus formation and apoptosis in transformed and nontransformed human cell lines.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

No statsitically significant effects in cell proliferation were observed in magnetic field exposed cells.
Magnetic field or tetradecanoylphorbol acetate (1nM) alone did not affect the number of micronuclei. Co-exposure (benzpyrene treatment for 24 hours during magnetic field exposure for 72 hours) resulted in an 1.8-fold increase of micronuclei formation compared to benzpyrene treatment for 48 hours alone. Co-exposure of benzpyrene, tetradecanoylphorbol acetate and magnetic field did not cause further micronuclei formation.
Since benzpyrene treatment (24 hours) during magnetic field exposure (72 hours) caused a significant increased micronuclei formation, these results suggest that magnetic fields enhance the initiation process of benzpyrene.

Study character:

Study funded by

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