Cells were additionally exposed to benzpyrene (as initiator) and/or tetradecanoylphorbol acetate (as cocarcinogen) plus/without extremely low frequency electromagnetic field in order to estimate carcinogenic/cocarcinogenic effects of extremely low frequency electromagnetic field.
Experiments were repeated three to five times.
Exposure duration: 24, 48, 72 h
|magnetic flux density||1 mT||-||measured||-||-|
No statsitically significant effects in cell proliferation were observed in magnetic field exposed cells.
Magnetic field or tetradecanoylphorbol acetate (1nM) alone did not affect the number of micronuclei. Co-exposure (benzpyrene treatment for 24 hours during magnetic field exposure for 72 hours) resulted in an 1.8-fold increase of micronuclei formation compared to benzpyrene treatment for 48 hours alone. Co-exposure of benzpyrene, tetradecanoylphorbol acetate and magnetic field did not cause further micronuclei formation.
Since benzpyrene treatment (24 hours) during magnetic field exposure (72 hours) caused a significant increased micronuclei formation, these results suggest that magnetic fields enhance the initiation process of benzpyrene.