Medical/biological study (experimental study)

8-Oxo-7, 8-dihydro-2'-deoxyguanosine as a biomarker of DNA damage by mobile phone radiation med./bio.

By: Khalil AM, Gagaa MH, Alshamali AM

Published in: Hum Exp Toxicol 2012; 31 (7): 734-740

Aim of study (acc. to author)

To study the effect of exposure to mobile phone 1800 MHz radiofrequency irradiation on the urinary level of 8-hydroxy-2-deoxyguanosine in adult male rats.

Background/further details

24 rats were divided into an exposure group and a sham exposure group (each group n=12). The exposure group was divided into four subgroups (each group n=3) and urine was collected at 0.5, 1, 2 and 4 hours after the beginning of exposure.

Endpoint

genotoxicity/mutation: DNA damage/oxidative stress (urinary level of 8-hydroxy-2-deoxyguanosine)

Exposure

- 1.8 GHz
- mobile communications
- mobile phone
- GSM

Exposure	Parameters
Exposure 1: 1,800 MHz Exposure duration: continuous for 2 h	electric field strength: 20 V/m maximum (e: electric field between 15 and 20 V/m d: Größe des elektrischen Feldes zwischen 15 und 20 V/m)

Exposure 1

Main characteristics

Frequency	1,800 MHz
Type	electromagnetic field
Exposure duration	continuous for 2 h

Exposure setup

Exposure source	antenna
Chamber	GSM signal generator located 2 cm besides the cage; antenna positioned to emit directly into the middle of the cage
Sham exposure	A sham exposure was conducted.

Parameters

Measurand	Value	Type	Method	Mass	Remarks
electric field strength	20 V/m	maximum	measured	-	e: electric field between 15 and 20 V/m d: Größe des elektrischen Feldes zwischen 15 und 20 V/m

Exposed system:

Methods Endpoint/measurement parameters/methodology

genotoxicity/mutation: DNA damage/oxidative stress (urinary level of 8-hydroxy-2-deoxyguanosine (adjusted to urinary creatinine level); ELISA)

Investigated system:

urine samples (supernatants)

Time of investigation:

after exposure

Main outcome of study (acc. to author)

Compared with the control group significant elevations in the levels of 8-hydroxy-2-deoxyguanosine in urine samples from exposed rats were found at all time points of urine collection (except at 0.5 h) with a maximum at 1 h after exposure suggesting DNA repair of DNA lesions leading to 8-hydroxy-2-deoxyguanosine formation.
The data suggest that radiofrequency exposure may augment oxidative stress and that the urinary level of 8-hydroxy-2-deoxyguanosine may be a good marker for oxidative stress.

Study character:

medical/biological study
experimental study
full/main study

Study funded by

Yarmouk University, Irbid, Jordan

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