Medical/biological study (experimental study)

The influence of 1800 MHz GSM-like signals on hepatic oxidative DNA and lipid damage in nonpregnant, pregnant, and newly born rabbits med./bio.

By: Tomruk A, Güler G, Dincel AS

Published in: Cell Biochem Biophys 2010; 56 (1): 39-47

Aim of study (acc. to author)

To study the possible biological effects of 1800 MHz GSM-like radiofrequency exposure on liver oxidative DNA damage and lipid peroxidation levels in non-pregnant and pregnant rabbits, and in their newborns.

Background/further details

18 non-pregnant and 18 pregnant rabbits were divided into four groups which were composed of nine rabbits: 1) non-pregnant control, 2) non-pregnant exposed rabbits, 3) pregnant control, 4) pregnant exposed animals. Newborns of the pregnant rabbits were also divided into two groups: 5) nine newborns of group 3 and 6) nine newborns of group 4 (intrauterine exposure during gestational period of day 15-22).

Endpoint

oxidative stress induced liver damage (hepato-toxicity: DNA damage, lipid peroxidation and malondialdehyde level)

Exposure

- PW pulsed wave
- mobile communications
- digital mobile phone
- GSM

Exposure	Parameters
Exposure 1: 1,800 MHz Modulation type: pulsed Exposure duration: continuous for 15 min/day on 7 days	power: 0.1 W

General information

animals were treated in six groups: i) non-pregnant control ii) non-pregnant exposed iii) pregnant control iv) pregnant exposed v) newborns of group iii) vi) newborns of gruop iv)

Exposure 1

Main characteristics

Frequency	1,800 MHz
Type	electromagnetic field
Exposure duration	continuous for 15 min/day on 7 days

Modulation

Modulation type	pulsed
Pulse width	0.576 ms
Duty cycle	12.5 %
Repetition frequency	217 Hz
Pulse type	rectangular

Exposure setup

Exposure source	horn antenna
Setup	horn antenna placed above the cage in a shielded room

Parameters

Measurand	Value	Type	Method	Mass	Remarks
power	0.1 W	-	-	-	-

Exposed system:

animal
rabbit/New Zealand White
whole body

Methods Endpoint/measurement parameters/methodology

genotoxicity/mutation: DNA damage in liver tissue (8-hydroxy-2-deoxyguanosine level)
oxidative stress induced liver damage: DNA damage (see "genotoxicity"), lipid peroxidation (measurement of oxidation of ferrous ions) and malondialdehyde level (spectrophotometry)

Investigated system:

DNA/RNA
liver homogenates

Investigated organ system:

liver

Time of investigation:

after exposure

Main outcome of study (acc. to author)

No significant differences were found in liver 8-hydroxy-2-deoxyguanosine levels of exposure groups (group 2 and 4) compared to the controls (group 1 and 3). However, in exposure groups malondialdehyde level and lipid peroxidation (ferrous ion oxidation) levels were increased compared to group 1. However, no significant difference was found in malondialdehyde level of group 4 (pregnant exposed) with respect to group 3 (pregnant control).
Remark EMF-Portal: Lipid peroxidation level of group 4 (pregnant, exposed) was not statistically significant different compared to group 1 (non-pregnant, control). Compared to group 3 (pregnant, control) the level of group 4 was statistically significant decreased.
No significant differences were found in liver tissue of 8-hydroxy-2-deoxyguanosine and malondialdehyde levels between the newborn groups (group 6 and group 5) while lipid peroxidation (ferrous ion oxidation) was significantly increased in group 6 with respect to group 5.
The authors conclude that whole body exposure to 1800 MHz GSM-like radiofrequency may lead to oxidative damage.

Study character:

medical/biological study
experimental study
full/main study
blind study

Study funded by

Gazi University Research Foundation, Turkey

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