研究のタイプ: 医学/生物学の研究 (experimental study)

[神経芽細胞の形態に対する50 Hz磁界ばく露の影響] med./bio.

Effect of 50 Hz magnetic field exposure on neuroblastoma morphology.

掲載誌: Int J Integr Biol 2007; 1 (1): 12-17

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研究目的(著者による)

The effects of an acute in vitro exposure of neuroblastoma cells to a 50 Hz magnetic field on differentiation and proliferation processes should be investigated.

詳細情報

Cell growth tests were repeated three times. Neurofilaments are components of the neural cytoskeleton und NF160 and NF200 are two common types.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 5 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 5 days
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cells were placed in 5 ml Corning flasks in the exposure area
ばく露装置の詳細 solenoid consisting of a 2 cm thick, 20 cm high asbestos cylinder with a diameter of 20 cm and 1200 turns of 2 mm diameter copper wire wound in three layers in continuous forward-backward fashion; solenoid placed inside a cell incubator, the centre of which was ventilated by a fan to guarantee a constant temperature; magnetic field homogenuos within 5% in the 11 cm x 17 cm exposure area along the solenoid's axis
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2 mT effective value 測定値 - -

Reference articles

  • Santoro N et al. (1997): [ヒトリンパ球系細胞株ラジ細胞への超低周波数磁界ばく露の形態学的、生物物理的影響]
  • Stauffer PR et al. (1994): [強磁性インプラントによる医療ハイパサーミアのための実用的誘導加熱コイルの構造]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Cell growth was significantly decreased in exposed cell cultures compared to sham exposure (comment from EMF-Portal editorial office: the graph shows an increase in cell growth - a contradiction). Moreover, morphology of exposed cells was strikingly altered by a stretched appearance with axon-like structures and blebs. Finally, higher levels of neurofilaments NF160 and NF200 could be observed in exposed cells via immunofluorescence tests.
The authors conclude that the results indicate that an acute in vitro exposure of neuroblastoma cells to a 50 Hz magnetic field decreases their growth rate and changes the cell morphology towards a neuronal cell like form. However, these results were not evaluated in any form.

研究の種別:

研究助成

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