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The effects of an acute in vitro exposure of neuroblastoma cells to a 50 Hz magnetic field on differentiation and proliferation processes should be investigated.
Cell growth tests were repeated three times. Neurofilaments are components of the neural cytoskeleton und NF160 and NF200 are two common types.
ばく露時間: continuous for 5 days
|ばく露時間||continuous for 5 days|
|チャンバの詳細||cells were placed in 5 ml Corning flasks in the exposure area|
|ばく露装置の詳細||solenoid consisting of a 2 cm thick, 20 cm high asbestos cylinder with a diameter of 20 cm and 1200 turns of 2 mm diameter copper wire wound in three layers in continuous forward-backward fashion; solenoid placed inside a cell incubator, the centre of which was ventilated by a fan to guarantee a constant temperature; magnetic field homogenuos within 5% in the 11 cm x 17 cm exposure area along the solenoid's axis|
|Sham exposure||A sham exposure was conducted.|
|磁束密度||2 mT||effective value||測定値||-||-|
Cell growth was significantly decreased in exposed cell cultures compared to sham exposure (comment from EMF-Portal editorial office: the graph shows an increase in cell growth - a contradiction). Moreover, morphology of exposed cells was strikingly altered by a stretched appearance with axon-like structures and blebs. Finally, higher levels of neurofilaments NF160 and NF200 could be observed in exposed cells via immunofluorescence tests.
The authors conclude that the results indicate that an acute in vitro exposure of neuroblastoma cells to a 50 Hz magnetic field decreases their growth rate and changes the cell morphology towards a neuronal cell like form. However, these results were not evaluated in any form.