Study type: Medical/biological study (experimental study)

60 Hz magnetic field exposure induces DNA crosslinks in rat brain cells. med./bio.

Published in: Mutation Research - Fundamental and Molecular Mechanism of Mutagenesis 1998; 400 (1-2): 313-320

Aim of study (acc. to author)

To study whether exposure to a 60 Hz magnetic field causes DNA-protein and DNA-DNA crosslinks in brain cells of the rat.

Background/further details

To study DNA-protein crosslinks, the slides in the microgel electrophoresis assay were treated with proteinase-K before electrophoresis which removes the protein from the DNA.
DNA is negatively charged and moves to the anode during electrophoresis. Most proteins crosslinked to DNA are positively charged and move to the cathode (thus, removal of protein would free the DNA and results in an increased DNA migration).
Some slides were additionally irradiated with X-rays (to identify DNA-DNA crosslinks).
The authors also compared the effects of magnetic fields with those of a known crosslink inducing agent (mitomycin C) (these experiment were performed in human lymphocytes).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 2 h

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration 2 h
Exposure setup
Exposure source
Chamber Plastic cage/ 45 cm x 21 cm wide x 22 cm high
Setup coils wound on frames fabricated from wood and aluminium; cage was placed in the center of the space between the coils
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT unspecified measured - -
power 8 W maximum - - at 1 mT exposure

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Investigated organ system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

These data indicate that both DNA-protein and DNA-DNA crosslinks are formed in brain cells of rats after acute exposure to a 60 Hz magnetic field. This is shown by the findings that magnetic field-induced DNA migration was only revealed after proteinase-K digestion, magnetic field exposure impeded X-ray-induced migration and proteinase-K treatment did not further increase X-ray-induced migration. This is further supported by the similarity to the effects of mitomycin C on lymphocyte DNA.

Study character:

Study funded by

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