Study type: Medical/biological study (experimental study)

Exposure of Daudi cells to low-frequency magnetic fields does not elevate MYC steady-state mRNA levels. med./bio.

Published in: Radiat Res 2000; 153 (5) Pt 2: 663-669

Aim of study (acc. to author)

To study the effects of low frequency magnetic field exposure on myc gene expression in Epstein-Barr virus (EBV)-positive Daudi cells.

Background/further details

The positive controls were perfomed with TPA treated cells.
EBV-positive Daudi cells were elected as cell line studied because it should be investigated whether there is a link between the magnetic field exposure and virus infection. Some investigators have suggested that conflicting results of studies of myc expression might be explained by the presence/absence of EBV infection.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 60 Hz
Exposure duration: 20, 40 or 60 min

Exposure 1

Main characteristics
Frequency 60 Hz
Type
Waveform
Exposure duration 20, 40 or 60 min
Exposure setup
Exposure source
Chamber inner exposure chamber 30 cm x 30 cm x 30 cm, double-walled, located within the coil system, insulated and foil-lined to shield from stray electric fields
Setup two Merritt coil sets nested into each other; the coils with 56 and 80 cm per side
Sham exposure A sham exposure was conducted.
Additional info two additional Helmholtz coils, 90 cm per side each, for the horizontally directed field
Parameters
Measurand Value Type Method Mass Remarks
magnetic flux density 500 µT maximum measured - -
magnetic flux density 100 µT - measured - -
magnetic flux density 50 µT - measured - -
magnetic flux density 12.5 µT - measured - -

Reference articles

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The exposure to a 60 Hz magnetic field did not significantly alter myc gene expression in Daudi cells under any of the exposure conditions.
No support for the hypothesis was found that EBV infection status plays a role in the sensitivity of cellular gene expression to electromagnetic field exposure was found.

Study character:

Study funded by

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