研究のタイプ: 医学/生物学の研究 (experimental study)

[卵母細胞の分化と卵胞の発育に対する低周波電磁界ばく露の影響] med./bio.

Effect of low-frequency electromagnetic field exposure on oocyte differentiation and follicular development.

掲載誌: Adv Biomed Res 2014; 3: 76

この研究は、BALB/cマウスを用いて、卵母細胞分化卵胞発育に対する低周波磁界(50Hz、3 mT)ばく露の影響を調べた。ばく露群(n=15)、擬似ばく露群(n=15)は、妊娠の全期間、1日4時間の磁界ばく露擬似ばく露)を受けた。両群から生まれた雌の新生仔の半分は出産時に、残りの半分は成獣に達した時点で、それぞれの卵母細胞を摘出して検査した。その結果、擬似ばく露群に比べ、ばく露群では、卵母細胞巣がほとんど破壊され、不規則に並んでいた;原始卵胞は生育が遅れ、電子顕微鏡像では卵母細胞の核の異質染色質と縮み、空胞化した細胞質が観察された、と報告している。

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研究目的(著者による)

To investigate the effects of extremely low frequency magnetic fields on the oocyte differentiation and follicular development in mice.

詳細情報

Pregnant mice were divided into an exposure and a sham exposure group (n=15 per group). Exposure/sham exposure took place during the pregnancy. After delivery, half of the female pups of both groups were sacrificed, while the other half was kept under normal conditions (no exposure) and sacrificed when they reached adulthood.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 4 hours/day during pregnancy (21 days)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 4 hours/day during pregnancy (21 days)
ばく露装置
ばく露の発生源/構造
  • 詳細不明
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 3 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Ovaries from exposed mice showed oocyte nests that were mostly broken and irregular arranged, while in the sham exposure group oocyte nests were intact and regularly arranged. Primordial follicles of mice from the exposure group were less developed than those from the sham exposure group and oocytes appeared to contain heterochromatin and were shrunken with vacuolated cytoplasm.
The authors conclude that extremely low frequency magnetic fields could influence the oocyte differentiation and follicular development in mice and that this may impair fertility.

研究の種別:

研究助成

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