この研究は、シスプラチン抵抗性ヒト卵巣腺がん細胞株A2780において、シスプラチン（30および60µg/ml：IC50は90µg/ml）投与および超低周波磁界ばく露（ELF-EMF: 50Hz、200ガウス、2時間ばく露）の単独あるいは組み合わせの処置による細胞毒性を調べた。その結果、ELF-EMFの単独ばく露は増殖を低下させた（<10 %）；30および60µg/mlのシスプラチンによる増殖速度低下は20および40%であった；ELF-EMFばく露を組み合わせると、シスプラチン抵抗性細胞のシスプラチン感受性が高まり、このような処置を受けた細胞ではp53発現が増加した、と報告している。
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It should be investigated if exposure to a 50 Hz magnetic field can overcome cisplatin resistance in human ovarian adenocarcinoma cells and initiate cell death at low concentrations of cisplatin.
Cells were divided into 6 groups: 1) treatment with 30 µg/ml cisplatin and exposure to the magnetic field, 2) 60 µg/ml cisplatin and MF exposure, 3) only MF exposure, 4) only treatment with 30 µg/ml cisplatin, 5) only treatment with 60 µg/ml cisplatin, 6) control group without exposure.
ばく露時間: for 2 hours
|ばく露時間||for 2 hours|
|ばく露装置の詳細||after exposure, cells were returned to an incubator for further 46 hours|
(remark EMF-Portal: significance of results becomes not clear and is contradictory in the article. Hence, no specifications on significance are reproduced in the following.)
Exposure to the magnetic field led to the reduction of cell viability in both co-exposure groups (groups 1 and 2) compared to exposure to the magnetic field alone (group 3), cisplatin alone (groups 4 and 5) or the control group (group 6).
Cells of group 1 showed a higher rate of DNA fragmentation, a higher amount of apoptotic cells and increased enzyme activities of caspases 3 and 9 compared to cells only exposed to the magnetic field (group 3), cisplatin (group 4) or the control group.
Gene expression of p53 was increased while gene expression of the matrix metalloproteinase-2 was decreased in group 1 compared to groups 3, 4 and 6.
The authors conclude that exposure to a 50 Hz magnetic field might sensibilize cisplatin-resistent human ovarian adenocarcinoma cells to low doses of cisplatin and thus enhance cell death.