研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波電磁界はp53活性化を介してシスプラチン抵抗性ヒト卵巣腺がん細胞の感受性を高める] med./bio.

Extremely low frequency electromagnetic field sensitizes cisplatin-resistant human ovarian adenocarcinoma cells via P53 activation.

掲載誌: Cytotechnology 2016; 68 (4): 1403-1413

この研究は、シスプラチン抵抗性ヒト卵巣腺がん細胞株A2780において、シスプラチン(30および60µg/ml:IC50は90µg/ml)投与および超低周波磁界ばく露(ELF-EMF: 50Hz、200ガウス、2時間ばく露)の単独あるいは組み合わせの処置による細胞毒性を調べた。その結果、ELF-EMFの単独ばく露増殖を低下させた(<10 %);30および60µg/mlのシスプラチンによる増殖速度低下は20および40%であった;ELF-EMFばく露を組み合わせると、シスプラチン抵抗性細胞のシスプラチン感受性が高まり、このような処置を受けた細胞ではp53発現が増加した、と報告している。

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研究目的(著者による)

It should be investigated if exposure to a 50 Hz magnetic field can overcome cisplatin resistance in human ovarian adenocarcinoma cells and initiate cell death at low concentrations of cisplatin.

詳細情報

Cells were divided into 6 groups: 1) treatment with 30 µg/ml cisplatin and exposure to the magnetic field, 2) 60 µg/ml cisplatin and MF exposure, 3) only MF exposure, 4) only treatment with 30 µg/ml cisplatin, 5) only treatment with 60 µg/ml cisplatin, 6) control group without exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: for 2 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 for 2 hours
ばく露装置
ばく露の発生源/構造
  • electromagnetic field generator
ばく露装置の詳細 after exposure, cells were returned to an incubator for further 46 hours
パラメータ
測定量 種別 Method Mass 備考
磁束密度 20 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

(remark EMF-Portal: significance of results becomes not clear and is contradictory in the article. Hence, no specifications on significance are reproduced in the following.)
Exposure to the magnetic field led to the reduction of cell viability in both co-exposure groups (groups 1 and 2) compared to exposure to the magnetic field alone (group 3), cisplatin alone (groups 4 and 5) or the control group (group 6).
Cells of group 1 showed a higher rate of DNA fragmentation, a higher amount of apoptotic cells and increased enzyme activities of caspases 3 and 9 compared to cells only exposed to the magnetic field (group 3), cisplatin (group 4) or the control group.
Gene expression of p53 was increased while gene expression of the matrix metalloproteinase-2 was decreased in group 1 compared to groups 3, 4 and 6.
The authors conclude that exposure to a 50 Hz magnetic field might sensibilize cisplatin-resistent human ovarian adenocarcinoma cells to low doses of cisplatin and thus enhance cell death.

研究の種別:

研究助成

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