細胞生存力／細胞分裂/増殖: neuronal differentiation: length of axons and distribution of neuronal markers: immunohistochemical stain of MAP2 (mature neuronal marker), DCX (immature neuronal marker) and c-Fos (marker for neuronal activity) in cells, fluorescence microscopy; Level of MAP2 and Tuj1 (mature neuronal marker) and DCX in cell lysate, Western blot
Both immunohistochemical staining and Western blot showed a significant increase in neuronal differentiation of cells in the exposure group compared to the sham exposure group. The differentiated neurons in the exposure group also showed significantly longer axons and a significantly increased level of c-Fos compared to the sham exposure group. Addition of the NMDA receptor inhibitor memantine reversed these effects. The authors conclude that exposure of human stem cells to a 50 Hz magnetic field could promote neuronal differentiation with the involvement of the NMDA receptor.