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To investigate the effect of a static magnetic field (SMF) on cell growth and gene expression in Escherichia coli.
ばく露時間: up to 50 h
|ばく露時間||up to 50 h|
|チャンバの詳細||Shaker incubator maintained at 37°C, 120 strokes per min and shielded with Faraday cage.|
|ばく露装置の詳細||The magnets were inserted into slits carved on a polystyrene plate and placed underneath the 50 ml cell culture flasks with the magnetic field vertically oriented|
|Sham exposure||A sham exposure was conducted.|
No change of cell growth could be found at cells cultured in LB-media. However, use of modified LB-media (containing glutamic acid and sodium chloride) leads to a significant increased growth of exposed cells.
Investigation of gene expression revealed altered expression patterns between exposed and no-exposed cells.