研究のタイプ: 医学/生物学の研究 (experimental study)

[バリア緊密性が高いインビトロモデルにおいて電磁界(GSM 1800)はスクロースに対する血液脳関門透過性を変えない] med./bio.

Electromagnetic fields (GSM 1800) do not alter blood-brain barrier permeability to sucrose in models in vitro with high barrier tightness.

掲載誌: Bioelectromagnetics 2005; 26 (7): 529-535

この研究は、GSM 1800標準の電磁界ばく露により、インビトロで作成された血液脳関門BBB)モデルを通過するスクロースの透過性が変化するか否かを調べた。著者らの先行研究は、ばく露による透過性亢進を報告したが、その時の実験で使用したBBBの細胞培養モデルは、ブタ脳の微小血管内皮細胞(PBEC)と共培養したラット星状細胞で構成されていた。今回の実験では、細胞培養条件の最適化を行って、バリア緊密性が明らかに向上した新しいBBBモデルを使用した。このBBBモデルの細胞培養物を、先行研究と同一のばく露システムで0.3 W / kgのSARで1〜5日間ばく露を実施した。その結果、細胞層を横断するスクロースの透過は、GSM 1800電磁界への最大5日間のばく露による影響を受けなかった;したがって、先行研究で報告したようなインビトロでのEMFばく露によるBBB透過性亢進は確認できなかった;今回の実験に用いたインビトロのBBBモデルの緊密性は、インビボの状態により近いものになっている、と報告している。

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研究目的(著者による)

The authors previously reported that electromagnetic fields (GSM 1800 standard, 1800 MHz) increased sucrose permeation across the blood-brain barrier in vitro (see publication 3911).
In this study the cell culture model was optimized (the filter inserts were changed) in a way that it now displays barrier properties that come very close to the low in vivo permeability.

詳細情報

Three different blood-brain barrier in vitro models were used. These models were comprised of 1) co-cultures of porcine brain capillary endothelial cells and rat astrocytes, 2) solitary porcine brain capillary endothelial cells cultivated in medium with addition of serum, and 3) without serum but with hydrocortisone.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1.8 GHz
Modulation type: pulsed
ばく露時間: continuous for up to 5 days
  • SAR: 0.3 W/kg mean
  • SAR: 0.46 W/kg maximum

ばく露1

主たる特性
周波数 1.8 GHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for up to 5 days
Modulation
Modulation type pulsed
Pulse width 0.577 ms
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • rectangular waveguide
ばく露装置の詳細 4-well dishes containing the cell cultures were placed into a waveguide for exposure and into another waveguide for sham exposure. The arrangement of the cells inside the waveguides was exactly as described in the reference article. Small holes were drilled in the waveguides to allow for the equilibration of the atmosphere required for the cell cultures. Both of these waveguides were located in an incubator. A control group of cell cultures was placed in the same incubator but outside the waveguides.
Additional information Since the waveguides were not designed to include a temperature probe, temperature control of the exposed cells was not possible.
パラメータ
測定量 種別 Method Mass 備考
SAR 0.3 W/kg mean 計算値 - -
SAR 0.46 W/kg maximum 計算値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In all three blood-brain barrier models, sucrose permeation across the cell layers remained unaffected by exposure to a 1800 MHz field for up to 5 days.The authors thus could not confirm enhanced permeability of the blood-brain barrier in vitro after electromagnetic field exposure as reported before since the in vitro barrier tightness in these experiments is now more like that of the in vivo situation.

研究の種別:

研究助成

Replicated studies

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