4-well dishes containing the cell cultures were placed into a waveguide for exposure and into another waveguide for sham exposure. The arrangement of the cells inside the waveguides was exactly as described in the reference article. Small holes were drilled in the waveguides to allow for the equilibration of the atmosphere required for the cell cultures. Both of these waveguides were located in an incubator. A control group of cell cultures was placed in the same incubator but outside the waveguides.
Since the waveguides were not designed to include a temperature probe, temperature control of the exposed cells was not possible.