研究のタイプ: 医学/生物学の研究 (experimental study)

[摘出された松果体が放出するメラトニンに対する1800 MHz電磁界の影響] med./bio.

1800 MHz electromagnetic field effects on melatonin release from isolated pineal glands.

掲載誌: J Pineal Res 2006; 40 (1): 86-91

ジャンガリアンハムスターから単離した松果腺メラトニン合成誘導するため、βアドレナリン作動性受容体作動薬イソプロテレノール刺激されたクレブス・リンゲル緩衝液によって灌流し、1800MHzの連続波(CW)もしくはSARが8、80、800、2700mW/kgのGSM-変調電磁波へ7時間ばく露した。実験は盲検で行った。灌流サンプルを毎時間回収し、メラトニン濃縮を放射免疫測定によって測った。CWおよびGSM両タイプの信号で、SARが800mW/lkgでメラトニンの放出は有意に高まったが、SARが2700mW/kgでは、CWではメラトニンレベルが上昇したが、GSMでは抑制された。SARが2700mW/kgで、温度が約1.2℃上昇するが、このレベルでの影響は熱的であった。RF電磁界について、非熱的ばく露メラトニン合成抑制することから、データは”メラトニン仮説”を裏付けないことがわかる。

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研究目的(著者による)

To study the direct effects of high-frequency 1800 MHz electromagnetic fields of different SAR levels on melatonin release by isolated pineal organs from Djungarian hamsters.

詳細情報

Both continuous wave and pulsed wave signals were used. The pulse signals were modulated according to the GSM.
The pineal glands were perifused with a special buffer containing the beta-adrenergic agonist isoproterenol to stimulate melatonin synthesis.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: CW
ばく露時間: continuous for 7 h
  • SAR: 8 mW/kg
  • SAR: 80 mW/kg
  • SAR: 800 mW/kg
  • SAR: 2,700 mW/kg
ばく露2: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for 7 h
  • SAR: 8 mW/kg
  • SAR: 80 mW/kg
  • SAR: 800 mW/kg
  • SAR: 2,700 mW/kg

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 7 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
チャンバの詳細 A modified radial waveguide exposure system was used whose principal features have been described in the reference article. Two identical waveguides (diameter 40 cm, height 3 cm) were placed in an incubator at 35 ± 1°C and activated one day prior to each experiment to allow for temperature equilibrium. Every experiment used one unit for exposure and one for sham-exposure controlled (blind randomized) by the data acquisition system.
ばく露装置の詳細 Inside the radial waveguides, which were terminated by thin absorber sheets (< -20 dB at 2 GHz), 20 glass cylinders each containing a pineal gland were arranged at a constant distance from a cone antenna in the center. The flexible tubes of the pumping system were connected to the glass receptacles through circular waveguides beyond cut-off to prevent any RF leakage. Each exposure unit contained one calibrated temperature probe instead of a pineal gland within a glass cylinder.
Additional information Different intensities for SAR values of 8, 80, 800, and 2700 mW/kg were used according to detailed calculations of the absorbed RF-EMF in the pineal gland tissue. The upper limit of 2700 mW/kg SAR was selected because pre-experiments revealed that higher values caused thermal effects (i.e. temperature differences of more than 1°C between exposed and control units).
パラメータ
測定量 種別 Method Mass 備考
SAR 8 mW/kg - 計算値 - -
SAR 80 mW/kg - 計算値 - -
SAR 800 mW/kg - 計算値 - -
SAR 2,700 mW/kg - 計算値 - -

ばく露2

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 7 h
Modulation
Modulation type pulsed
Additional information

GSM-modulated

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 8 mW/kg - 計算値 - -
SAR 80 mW/kg - 計算値 - -
SAR 800 mW/kg - 計算値 - -
SAR 2,700 mW/kg - 計算値 - -

Reference articles

方法 影響評価項目/測定パラメータ/方法

研究対象とした臓器系:
調査の時期:
  • ばく露中

研究の主なアウトカム(著者による)

Both types of signal significantly enhanced melatonin release at 800 mW/kg, while at 2700 mW/kg melatonin levels were elevated in the continuous wave, but suppressed in the GSM-exposed pineal glands. As a temperature rise of approximately 1.2°C was measured at 2700 mW/kg, effects at this level are thermal.

研究の種別:

研究助成

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