研究のタイプ: 医学/生物学の研究 (experimental study)

[実験的白質脱髄モデルにおいて電磁界刺激は脳室下帯神経幹細胞の回復による内因性ミエリン修復を高める] med./bio.

Electromagnetic field stimulation potentiates endogenous myelin repair by recruiting subventricular neural stem cells in an experimental model of white matter demyelination.

掲載誌: J Mol Neurosci 2012; 48 (1): 144-153

この研究は、ラットにおける毒素誘導性の脱髄と髄鞘再生に対する磁界(60 Hz; 0.7 mT)の影響を調べた。実験には30匹の雌SDラットを用いた。脱随を誘導するための脳梁内にリゾホスファチジルコリン(LPC)注射を行った後、ばく露群と擬似ばく露群に分け、処置日から7、14、28日間、2時間の磁界ばく露(または擬似ばく露)を1日2回(午前と午後)行い、各ばく露期間終了後直ちに屠殺して、ネスチンとミエリン塩基タンパク質(MBP)のBrdUラベリングおよび免疫染色により骨髄組織検査を行った。その結果、14、28日間のばく露群では脱随面積の減少と脱随部位内のMBP染色レベル増加が有意であった;7、14日間のばく露群では脳室下帯とLPC注入傷痕の間の部位でのBrdU陽性細胞、ネスチン陽性細胞の数が増加したとの所見から、ばく露群では神経細胞増殖ミエリン鞘修復の増強がみられたと報告している。

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研究目的(著者による)

The effects of exposure of multiple sclerosis model rats to a 60 Hz magnetic field on proliferation and migration of neural stem cells and repair of myelin in the corpus callosum should be investigated.

詳細情報

Multiple sclerosis was induced in rats by an injection of lysophosphatidylcholine into the corpus callosum. Neural stem cells are capable to form new myelin sheats and might therefore be of use in the treatment of multiple sclerosis.
30 rats were divided into the following groups (n ≥ 3 each): 1) sham injection with salt solution and exposure to the magnetic field (remark EMF-Portal: it is not clear how long this group was exposed), multiple sclerosis model rats and exposure to the magnetic field for 2) 7 days, 3) 14 days and 4) 28 days. For each group a separate sham exposure group was used.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 2 x 2 per day for 7 days
ばく露2: 60 Hz
ばく露時間: continuous for 2 x 2 per day for 14 days
ばく露3: 60 Hz
ばく露時間: continuous for 2 x 2 per day for 28 days

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 2 x 2 per day for 7 days
Additional information transcranial electromagnetic field stimulation, oscillatory magnetic field
ばく露装置
ばく露の発生源/構造
チャンバの詳細 plastic cylindrical restrainers
ばく露装置の詳細 a pair of 7-cm-diameter Helmholtz coils was located dorsal and ventral of the head; the distance between each coil and the midpoint of the head was approximately 6 cm
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.7 mT - - - -

ばく露2

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 2 x 2 per day for 14 days
Additional information transcranial electromagnetic field stimulation, oscillatory magnetic field
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.7 mT - - - -

ばく露3

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 2 x 2 per day for 28 days
Additional information transcranial electromagnetic field stimulation, oscillatory magnetic field
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.7 mT - - - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Exposure to the magnetic field significantly reduced the extent of myelin destruction in the corpus callosum of multiple sclerosis model rats after 14 (group 3) and 28 days (group 4) of exposure compared to the respective sham exposure groups. Corresponding to that, the presence of Schwann cells was significantly increased in rats from all exposure groups (groups 2-4) compared to sham exposed rats.
The number and proliferation of neural stem cells in the corpus callosum was significantly increased in all exposure groups compared to the sham exposure groups, indicating a migration of neural stem cells to the damaged areas and cell growth.
The authors conclude that exposure to a 60 Hz magnetic field might promote a proliferation and migration of neural stem cells and repair of myelin in damaged tissue of the corpus callosum in multiple sclerosis model rats.

研究の種別:

研究助成

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