研究のタイプ: 医学/生物学の研究 (experimental study)

[卵巣摘出術および長期間の超低周波磁界ばく露の後でラット下顎骨関節突起に組織学的および組織形態計測的変化が引き起こされるか?] med./bio.

Can histological and histomorphometrical changes be induced in rat mandibular condyle following ovariectomy and long-term extremely low frequency magnetic field exposure?

掲載誌: Biotechnol Biotechnol Equip 2012; 26 (2): 2916-2920

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研究目的(著者による)

To investigate whether histopathological changes of the mandibular condyle structure occur in ovariectomized rats exposed to extremely low frequency magnetic fields.

詳細情報

Female rats were divided into four groups (n=10 per group): 1.) control group, 2.) ovariectomy, 3.) exposure and 4.) ovariectomy + exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 4 h/day during 6 months

General information

rats were divided into the following four groups: i) control ii) ovariectomy (sham exposure) iii) EMF exposure iv) ovariectomy + EMF exposure

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 h/day during 6 months
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 two pairs of Helmholtz coils with a diameter of 70 cm and 125 turns of insulated soft copper wire with a diameter of 1.5 mm, coils placed vertically at a distance of 47 cm inside a 130 cm x 65 cm x 80 cm Faraday cage; rats kept in 43 cm x 42 cm x 15 cm methacrylate boxes
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.5 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In all experimental groups (groups 2-4), some histopathological changes such as irregular appearance in bone marrow cavities were observed, while the structure in the control group appeared normal. In the ovariectomized rats (groups 2 and 4), a loss in bone marrow was determined, while there was an increased amount of bone marrow in the rats exposed to magnetic field alone (group 3). In both magnetic field exposed groups (groups 3 and 4), a disruption and latency in the calcification and ossification was observed. However, no significant differences occurred in the thickness of the condylar cartilage layers between the groups.
The authors conclude that long-term exposure to extremely low frequency magnetic fields and the ovariectomy could induce histopathological changes in the rat's mandibular condyle. However, magnetic field exposure had no influence on the ovariectomy-induced changes.

研究の種別:

研究助成

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