研究のタイプ: 医学/生物学の研究 (experimental study)

[イン・ビトロで2450MHz無線周波放射にばく露したヒト血液リンパ球における増殖および細胞遺伝学的研究] med./bio.

Proliferation and cytogenetic studies in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation.

掲載誌: Int J Radiat Biol 1997; 72 (6): 751-757

この研究は、2人の健常人ボランティアから採取したヒト末梢血の標本に、インビトロで、2450MHz高周波(RFR)ばく露を与え、その影響を調べた。ばく露は、90分間の連続または総ばく露時間90分間の断続(30分オン、30分オフの3回繰り返し)。擬似ばく露群およびガンマ線照射群(インビトロで150cGy照射された末梢血標本)を対照群として用いた。連続波2450MHzのRFRは、垂直下方に向けられた標準ゲイン矩形ホーンアンテナから順正味出力34.5Wで送信された。細胞の位置における平均電力密度は5.0mW / cm 2であった。平均SARは12.46W / kgと計算された。リンパ球は、ばく露直後に、48時間および72時間培養され、それぞれ染色体異常および小核の出現頻度が測定された。増殖指数も記録された。その結果、RFRの連続および断続的なばく露のどちらにおいても、ばく露群と擬似ばく露群の間で、以下のものに有意差はなかった。(a)有糸分裂指数、(b)染色体損傷を示す細胞の発生率、(c)交換染色体異常、(d)無動原体断片、(e)二核リンパ球、(f)小核;対照的に、150cGyガンマ線ばく露を受けた陽性対照群の反応は、RFRばく露および擬似ばく露群とは有意に異なっていた;したがって、2450MHzのRFRにインビトロばく露されたヒト血液リンパ球において、マイトジェン活性化による増殖の速度論または72時間以内の遺伝毒性過剰への影響についての証拠はない、と報告している。

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研究目的(著者による)

To study the effects of in vitro exposure to continuous wave 2450 MHz radiofrequency irradiation on mitogenic stimulation, proliferation kinetics and the extent of cytogenetic damage in human blood lymphocytes.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2,450 MHz
Modulation type: CW
ばく露時間: continuous for 90 min
ばく露2: 2,450 MHz
Modulation type: CW
ばく露時間: intermittent, 30 min on/30 min off, for 90 min

General information

For each exposure time pattern, nine 6-ml aliquots of whole blood were distributed into separate T-25 tissue culture flasks, three of which were RF exposed, three were sham-exposed, and three were positive controls.

ばく露1

主たる特性
周波数 2,450 MHz
タイプ
  • electromagnetic field
特性
  • near field
ばく露時間 continuous for 90 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
Distance between exposed object and exposure source 1.75 m
チャンバの詳細 The anechoic room measuring 18.0 x 9.0 x 9.0 ft was maintained at 37 ± 1°C. A pressed-foam incubator box measuring 36 x 27 x 13.5 cm was placed directly under the antenna horn and was gently rocked side-to-side.
ばく露装置の詳細 Three T-25 tissue culture flasks containing 6 ml of whole blood and a fourth flask containing 6 ml of tissue culture medium (for temperature monitoring) were placed in separate impressions cut into the underside of four plugs fitting into the top of the incubator box. The cells at the bottom of the culture flasks were at 1.75 m from the antenna opening. They were equilibrated at 37°C before exposure, and the temperature increase during exposure was restricted to below 39°C.
Sham exposure A sham exposure was conducted.
Additional information The identical incubator box for sham exposure was placed at the far end of the anechoic room and was also gently rocked. The flasks for positive control were placed next to the sham exposure box and remained stationary. At the end of the exposure period, they were immediately exposed in vitro (at room temperature) to an acute dose of 150 cGy of 137Cs gamma radiation at a dose rate of 108.7 cGy/min.
パラメータ
測定量 種別 Method Mass 備考
電力密度 5 mW/cm² mean 測定値 - ±7%
SAR 12.46 W/kg mean 計算値 - ± 0.1 W/kg

ばく露2

主たる特性
周波数 2,450 MHz
タイプ
  • electromagnetic field
特性
  • near field
ばく露時間 intermittent, 30 min on/30 min off, for 90 min
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 5 mW/cm² mean 測定値 - ±7%
SAR 12.46 W/kg mean 計算値 - ± 0.1 W/kg

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

There were no significant differences between exposed and sham-exposed lymphocytes with respect to mitotic indices, incidence of cells showing chromosome damage, exchange aberrations, acentric fragments, binucleated lymphocytes, and micronuclei, for either the continuous or intermittent radiofrequency exposures. Thus, there is no evidence for an effect on mitogen-stimulated proliferation kinetics or for excess genotoxicity within 72 h in human blood lymphocytes exposed to 2450 MHz radiofrequency irradiation.

研究の種別:

研究助成

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